| Sequence: |
5’-tcgtcgttttgtcgttttgtcgtt-3’(lower case letters indicate phosphorothioate linkage). |
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| MW: |
7720 (ammonium salt) |
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| Source: |
Synthetic. |
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| Quantity: |
12.5nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate). |
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| Formulation: |
Lyophilized. Sterile. |
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| Purity Detail: |
Activity and endotoxin tested - TLRGRADE™. |
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| Endotoxin Content: |
<0.002 EU/µg (LAL test; BioWhittaker) |
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| Reconstitution: |
For a 100µM stock solution, dissolve the total vial content in 125µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving. |
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| Shipping: |
Ambient |
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| Long Term Storage: |
+4°C |
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| Use/Stability: |
Aqueous stock solution is stable for 1 day when stored at +4°C. |
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| Handling: |
Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: |
Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For the inactive control compound, please see Prod. No. ALX-746-206. |
