DL-Дитиотреитол ≥99.0%

DL-Dithiothreitol

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DL-Dithiothreitol is a pivotal component used in the preparation of buffer which in turn is utilized in the platelet cytosol preparation.^[2] It is also important in isolation of RNA by using guanidinium salts and homogenisation of tissue^[1] and cells^[3].

 

Информация для заказа

Область использования: Реагенты Sigma Aldrich	Производство:	Sigma
	Метод:
	Объем:	5 г.
	Кат. номер:	43819-5G
Цена (с НДС 20%):	по запросу	В корзину
Наименование: DL-Дитиотреитол ≥99.0% / DL-Dithiothreitol. Примечание: