Набор Annexin V-PE Apoptosis Detection, суспензии клеток

Annexin V-PE Apoptosis Detection Kit

• Product nameAnnexin V-PE Apoptosis Detection Kit
  See all Annexin V kits
• Tests
  100 x 1 test
• Sample type
  Adherent cells, Suspension cells
• Assay typeDirect
• Assay time
  0h 10m
• Product overview

  Annexin V-PE Apoptosis Detection Kit (ab14155) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
  Visit our FAQs page for tips and troubleshooting.

• Tested applicationsFluorescence Microscopy, Flow Cytmore details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	100 tests
  Annexin V-PE	1 x 500µl
  Binding Buffer	1 x 50ml

• FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
• Involvement in diseasePregnancy loss, recurrent, 3
• Sequence similaritiesBelongs to the annexin family.
  Contains 4 annexin repeats.
• DomainThe [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  A pair of annexin repeats may form one binding site for calcium and phospholipid.
• Post-translational
  modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
• Information by UniProt
• Alternative names
  • Anchorin CII
  • Annexin 5
  • Annexin A5

   

  Applications

  Our Abpromise guarantee covers the use of ab14155 in the following tested applications.

  The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  Application	Abreviews	Notes
  Fluorescence Microscopy		Use at an assay dependent concentration.
  Flow Cyt		Use at an assay dependent dilution.

  Annexin V-PE Apoptosis Detection Kit images

  • 

    Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)

    Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H₂O₂ (Sigma) for 6 hours (red).

  Protocols

  • Protocol Booklet

Информация для заказа

Область использования: Диагностические наборы для проточной цитометрии	Производство:	Abcam
	Метод:	Проточная цитометрия
	Объем:	100 тестов
	Кат. номер:	ab14155
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Набор Annexin V-PE Apoptosis Detection, суспензии клеток / Annexin V-PE Apoptosis Detection Kit. Примечание: +4°C Дополнительная информация (на английском).