CD326 (EpCAM)-PE, human (clone: HEA-125)
Clone HEA-125 recognizes CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA) and is involved in cell adhesion. The CD326 antigen is broadly expressed on the basolateral surface of carcinoma and epithelial cells in tissues or on circulating tumor cells, and cancer stem cells, but is not found on melanoma, neuroblastoma, sarcoma, lymphoma, leukemia cells, or normal fibroblasts.
||Mouse IgG1 – isotype control antibodies
|Alternative names of antigen
||EGP40, Ep-CAM, KSA, MK-1, TROP1
|Entrez Gene ID
|Molecular mass of antigen [kDa]
|Distribution of antigen
||cancer stem cells, epithelial cells, lung, ES and iPS cells
||Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
||The antibody is suited for staining of formaldehyde-fixed cells.
||Store protected from light at 2–8 °C. Do not freeze.
||FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, Biotin
Peripheral blood leukocytes mixed with cells from a breast cancer cell line (SK-BR-3) were stained with CD326 (EpCAM) antibodies conjugated to (A) FITC, (B) PE, (C) APC, (D) VioBlue, (E) APC-Vio770, or (F) Biotin and analyzed by flow cytometry using the MACSQuant® Analyzer. FcR Blocking Reagent (# 130-059-901) was added to block unwanted binding of antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or DAPI fluorescence as in the case of tandem-conjugates.
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