| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Cell Isolation and Identification |
| Category ID_II | Cell Identification Kits |
| Category ID_III | Cell Identification Kits |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
Morphological observations of RAW 264.7: untreated RAW 264.7 (left) cells show a round or oval morphology. RAW 264.7 Differentiated M1 macrophages (right) show irregular morphology and contain granular material in the cytoplasm.
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Phenotypic analysis of M1 macrophages of RAW 264.7: RAW 264.7 cells were induced by polarization with RAW 264.7 Cell M1 Differentiation MIX (1000×) for 48h (M1-48h) and unpolarized treated blank RAW 264.7 cells (control-48h) were cultured for 48h with flow cytometry Detection and analysis.
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Cytokine expression analysis of M1 macrophages of RAW 264.7: RAW 264.7 cells were induced by polarization using RAW 264.7 Cell M1 Differentiation MIX (1000×) for 48h (M1-48h) and unpolarized blank RAW 264.7 cells (control-48h) were cultured for 48h and the supernatants were collected, and the supernatants were analyzed using the Mouse TNF-alpha (Tumor Necrosis Factor Alpha (Tumor TNF-α) ELISA Kit(E-EL-M3063)/Mouse IL-12 (Interleukin 12) ELISA Kit(E-EL-M3062)/Mouse IL-6 (Interleukin 6) ELISA Kit(E-EL-M0044) /Mouse IL-1β(Interleukin 1 Beta) ELISA Kit(E-EL-M0037) detects and analyzes the results.
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| Application | FCM |
| Usage | This kit provides a complete set of differentiation and identification protocols for RAW 264.7 polarized to M1 macrophages, including M1 macrophage differentiation differentiation and identification reagents, which provides stable and reliable support for the differentiation and identification of RAW 264.7 polarized to M1 macrophages, and improves the efficiency and stability of differentiation and identification of RAW 264.7 polarized to M1 macrophages. |
| Tested Sample Types | RAW 264.7 Cells |
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