| Storage | This product can be stored at 2~8℃ for 1 year in the dark. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Apoptosis |
| Category ID_III | Caspase Assay Kits |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
| MSDS | ссылка |
Control: Normal Hela cells were not treated with DMSO.
10% DMSO-4h: Hela cells were treated with 10% DMSO for 4h.
HeLa cells stained with Caspase 3/7 Substrates (green) and DAPI Reagent (25 μg/mL) (blue).
 |
 |
| Detection Instrument | Flow Cytometer;Fluorescence Microscope |
| Tested Sample Types | Cell samples |
| Assay Time | 0.5 hour |
| Detection Type | Fluorometric method;Flow cytometry |
| Test Principle | The Caspase 3/7 Substrates（Green）are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously.
Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA.
DAPI co-staining of cells with Caspase 3/7 Substrates allows simultaneous differentiation of late-apoptotic cells, necrotic cells, and caspase 3/7 enzyme activity during different apoptotic periods. |
| | |
