| Storage | This product can be stored at 2~8℃ for 1 year in the dark. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Apoptosis |
| Category ID_III | Caspase Assay Kits |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
| MSDS | ссылка |
Control: Normal Hela cells were not treated with DMSO.
10% DMSO-4h: Hela cells were treated with 10% DMSO for 4h.
HeLa cells stained with Caspase 3/7 Substrates (green) and Propidium Iodide (PI) Solution (750 μM) (red).
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| Detection Instrument | Flow Cytometer;Fluorescence Microscope |
| Tested Sample Types | Cell samples |
| Assay Time | 0.5 hour |
| Detection Type | Fluorometric method;Flow cytometry |
| Test Principle | The Caspase 3/7 Substrates（Green）are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously.
Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA, and emitting red fluorescence.
PI co-staining of cells with Caspase 3/7 Substrates allows simultaneous differentiation of late-apoptotic cells, necrotic cells, and caspase 3/7 enzyme activity during different apoptotic periods. |
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