| Storage | This product can be stored at -20℃ for 1 year in the dark. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Apoptosis |
| Category ID_III | Caspase Assay Kits |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
| MSDS | ссылка |
Jurkat cells were stained with Caspase 9 Substrates (Green) and analyzed by flow cytometry.
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Hela cells were culture with (right) or without (left) 5% DMSO for 3.5 h and then stained with Caspase 9 Substrates (Green) and photographed after fluorescence microscopic observation.
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| Kit Type | Apoptosis/Caspase
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| Detection Instrument | Flow Cytometer;Fluorescence microscope
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| Tested Sample Types | Cell samples
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| Assay Time | 30 min |
| Detection Type | Fluorometric method;Flow cytometry |
| Test Principle | The Caspase 9 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 9 recognition sequence (LEHD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-9 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-9 activity and visualizing the morphological changes of the cell nucleus during apoptosis. |
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