| Storage | This product can be stored at -20℃ for 1 year in the dark. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Apoptosis |
| Category ID_III | Caspase Assay Kits |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
| MSDS | ссылка |
RAW264.7 cells were stained with Caspase 1 Substrates (Green) and analyzed by flow cytometry.
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RAW264.7 cells were stained with Caspase 1 Substrates (Green) and photographed after fluorescence microscopic observation.
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THP-1 cells were induced by LPS at different concentrations (0 μg/mL , 1 μg/mL), and Caspase 1 Substrates (Green) were added to detect the dynamic changes of caspase1 enzyme activity in LPS-induced cells at different time points of 4h/6h/24h.
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| Kit Type | Apoptosis/Caspase |
| Detection Instrument | Flow Cytometer;Fluorescence microscope |
| Tested Sample Types | Cell samples |
| Assay Time | 30 min |
| Detection Type | Fluorometric method;Flow cytometry |
| Test Principle | The Caspase 1 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 1 recognition sequence (YVAD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-1 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-1 activity and visualizing the morphological changes of the cell nucleus during apoptosis. |
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