| Storage | This product can be stored for 1 year at -20°C |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Function Related Products |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
| MSDS | ссылка |
Jurkat cells were incubated and cultured in medium with (10 mM 2-DG (2-Deoxy-D-glucose)) or without 2-DG (Untreated control) for 60 min, washed and then incubated with 2-NBDG Glucose Uptake Assay Kit for 60 min, and flow cytometric detection of The glucose uptake of the cells was detected by flow cytometry. Incubation with 2-DG inhibited the glucose uptake of Jurkat cells.
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Hela cells were incubated and cultured with (left) or without 10 mM 2-DG (right) for 60 min, washed and then incubated with 2-NBDG Glucose Uptake Assay Kit for 60 min, and glucose uptake was detected by fluorescence microscopy, and glucose uptake of Hela cells was inhibited by the addition of 2-DG incubation.
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| Usage | Can be used for flow cytometry and fluorescence microscopy to detect the glucose uptake viability of cells; can also be used for the evaluation and screening of drugs for glucose metabolic diseases |
| Kit Type | Cell Function Related Products |
| Detection Instrument | Fluorescence microscope;flow Cytometer |
| Tested Sample Types | Cell samples |
| Assay Time | 2 hours |
| Detection Type | Fluorometric method; Flow cytometry |
| Test Principle | 2-NBDG Reagent is a green fluorescently labeled deoxyglucose analog with the same transporters (GLUTs) and similar transport kinetics as D-glucose.It is allowed to be phosphorylated by hexokinase because of a modification at the C-2 position.When it enters the cell, it is phosphorylated by hexokinase at the C-6 position to produce 2-NBDG-6-phosphate (a fluorescent derivative). Thus the fluorescence intensity reflects the glucose uptake. |
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