| Storage | This product can be stored at -20°C for 12 months with shading light. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Proliferation and Cycle |
| Category ID_III | CFSE Cell Proliferation Assay Kits |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
The mouse bone marrow-derived dendritic cells cultured in vitro were matured by LPS, and co-cultured withthe spleen of 4T1 tumor-bearing mice (E-CK-A345 staining). After co-staining with Elab Fluor<sup>®</sup> Red 780Anti-Mouse CD8a Antibody (E-AB-F1104S) and APC Anti-Mouse CD4 (E-AB-F1097E), detect theproliferation of CD8+T cells.
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Human peripheral blood mononuclear cell (PBMC) were stimulated with Human T-Activator CD3/CD28, then stained with CFSE Cell Division Tracker Kit on Day 0 (empty black histogram) or on Day 7 (filled gray histogram). Solid gray peaks represent successive generations.
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| Kit Type | Cell Proliferation |
| Detection Instrument | Flow Cytometer;Fluorescence Microscope |
| Tested Sample Types | Cell samples |
| Detection Type | Fluorometric method |
| Test Principle | CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps. |
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