| Storage | This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Apoptosis |
| Category ID_III | Mitochondrial Assay Kits |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
| MSDS | ссылка |
The effect of this kit to detect apoptosis induced by camptothecin in Jurkat cells is shown: normal cells (left) have a small amount of apoptosis, which is manifested as a small amount of mitochondrial membrane potential collapse cells; induced apoptotic cells (middle, 2.5 μM camptotheca Alkaline-treated Jurkat cells for 24 h) had a large number of mitochondrial membrane potential collapse cells; CCCP-treated cells (right, positive control) almost all cells had mitochondrial membrane potential collapse.
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Normal Hela cells(Control,top) were not treated with drugs. have a small amount of apoptosis, which is manifested as a small amount of mitochondrial membrane potential collapse cells; 10 μM camptotheca treated Hela cells for 24 h (bottom) , had a large number of mitochondrial membrane potential collapse cells.
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Normal Hela cells(Control,top) were not treated with drugs,have a small amount of apoptosis, which is manifested as a small amount of mitochondrial membrane potential collapse cells; 10 μM CCCP treated Hela cells for 40 min (bottom) , had a large number of mitochondrial membrane potential collapse cells.
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| Kit Type | Apoptosis/JC-1 |
| Detection Instrument | Flow Cytometer;Fluorescence Microscope |
| Tested Sample Types | Cell samples |
| Assay Time | 40min |
| Detection Type | Fluorometric method;Flow cytometry |
| Test Principle | The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic features (chromatin condensation and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible.
JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ?Ψm. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t accumulate in the mitochondrial matrix. When JC-1 is a monomer, it can produce green fluorescence.
The decrease of cell membrane potential can be detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition of JC-1 fluorescence color can be used as an early detection indicator of cell apoptosis. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization.
The maximum excitation wavelength of JC-1 monomer is 514 nm and the maximum emission wavelength is 529 nm; the maximum excitation wavelength of JC-1 polymer is 585 nm and the maximum emission wavelength is 590 nm. |
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