| Storage | This product can be stored at -20°C for 12 months with shading light. |
| shipping | Ice bag |
| Exp date | 12 months |
| Category ID_I | Apoptosis and Cell Health Detection |
| Category ID_II | Cell Cytotoxicity and Viability |
| Category ID_III | Calcein AM/PI Double Staining Kit |
| Описание на сайте производителя | ссылка |
| Инструкция | ссылка |
| MSDS | ссылка |
4T1 cells and Hela cells were treated with 5 % DMSO, 5μM Camptothecin, 10μM Camptothecin for 4h, then stained with Calcein-AM / PI Double Staining Kit and photographed.
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Molt-4 cells were treated with 5 % DMSO and 10 % DMSO for 4h, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.
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Jurkat cells were placed at 4 °C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.
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| Kit Type | Viability/Cytotoxicity |
| Detection Instrument | Flow Cytometer;Fluorescence Microscope |
| Tested Sample Types | Cell samples |
| Assay Time | 30 min |
| Detection Type | Fluorometric method;Flow cytometry |
| Test Principle | PI is a kind of nucleic acid dye. Due to the loss of cell membrane selective permeability of dead cells, Propidium Iodide (PI) can enter the cell and specifically bind with double-stranded DNA and produce red fluorescence (Ex/Em=535nm/617nm), thus marking dead cells. |
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