EZcount™ ATP Cell Assay Kit
Introduction
Cell proliferation and death are ATP-dependent essential processes for tissue generation and regeneration, organ development etc. in mammals and are usually under stringent control of extra and intracellular factors. Non-physiological alterations in levels of these factors lead to anomalous cytogenetic behavior of cells which in turn leads to cell transformation, uncontrolled cell growth - the initiating event for cancer development. Pharmaceutical research is hence largely focused on effects of drugs, cytotoxic agents and biologically active compounds which affect cytogenetics. The cellular ATP content plays an important role in determining the fate of a cell. A fairly constant level of ATP in cells maintains them in viable state, or otherwise leads to apoptosis. Multiple procedures are available for determination of cell proliferation and cytotoxicity. Simple and cheap methods for estimating cell viability (or death) are Trypan Blue exclusion and Erythrocin B staining. However, these methods are not sensitive enough and cannot be used for high throughput screening. Measuring the uptake of radioactive substances, usually tritium-labeled thymidine, is accurate but it is also time-consuming and involves handling of radioactive substances. The ATP-based cell assay is a simple, accurate and reproducible assay for determining cellular proliferation and cytotoxicity. The results can be read on a luminometer.
About the Assay
The EZcountTM ATP Cell Assay Kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agents. The kit is based on the generation of luminescence as ATP catalyzes the reaction between D-luciferin and enzyme luciferase. The reaction is as follows
In the above reaction, the reaction between enzyme luciferase and D-luciferin is dependent on presence of ATP. ATP is ubiquitously present in all viable, metabolically active cells. On the other hand, cell death is characterized by a significant decrease in the ATP level.
Applications
• Toxicity of physical agents either alone or in
combination with chemicals and in particular
phototoxicity
• Antibody dependent complement cell damage
• Tumoral cell growth and chemotherapy
effectiveness
• Ranking chemicals according to their toxic
potencies or hazard
• Toxicity prevention and treatment effectiveness
• The investigation of metabolism mediated
cytotoxicity (detoxification and toxification
process)
• The study of temperature-dependent toxicity
Kit contents
The reagents supplied in this kit are sufficient for 1000
assays (for ten 96 well plates).
Materials required but not provided in the
kit
• Ca2+ and Mg2+ free PBS (TL1006)
• Cells in appropriate medium
• Adjustable pipettes and a repeat pipettor
• Flat-bottom 96-well microtiter plate (white) for
culturing the cells
• Luminometer
Информация для заказа
| Область использования: | Производство: | HIMEDIA |
| Метод: | |
| Объем: | 1000 тестов |
| Кат. номер: | CCK051-1000 |
| Цена (с НДС 20%): | по запросу | В корзину  |
Наименование: EZcount™ ATP Cell Assay Kit.
Примечание: Cell viability kits based on Cellular ATP content |
