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C16H20N2·2HCl F.W. 313.30 CAS #64285-73-0
Also available in tablet form. 1mg/ tablet, ready to use.
(1,4-Butanediol dimethacrylate)
Si(CH3)4 M.W. 88.22 An organo-silicon compound tetramethylsilane (TMS) is used as an internal standard for nuclear magnetic resonance (NMR). It is chemically inert and has a boiling point of 26.6°C which is lower than acetone (53.6°C), and other highly volatile liquids, such as diethyl ether (34.5°C) and 1,2-epoxy propane (36.4°C). It is soluble in most organic solvents but insoluble in water. All of these properties make TMS an ideal solvent for tissue drying, which preserves excellent tissue surface details. Dey, Sudip. Et al. (1989). A new rapid method of air-drying for scanning electron microscopy using tetramethylsilane. J. of Microscopy, Vol 156, pp. 259-261.
(2,2'-Di[p-nitrophenyl]-5,5'-di[p-thiocarbamylphenyl] -3,3'-[3,3'-dimethoxy-4,4'-biphenylene]ditetrazolium chloride)
|
RT | 21900 | 5g |
NH2CSNHNH2 F.W. 91.14 CAS #79-19-6
m.p. 181-183°C
Assay >99.0%
References for use-see Thiocarbohydrazide
RT | 22000 | 25g |
Please see listing in General Supplies for Histology.
C6H5CH3 FW 92.14 CAS #108-88-3
Specific Gravity (H2O=1): 0.866
b.p. 110-111°C (230-222°F)
Assay. 99.9%
Color (A.P.H.A.) | 10 |
Residue after evaporation | 0.001% |
Subs. darkened by H2SO4 | To pass test |
Sulfur compound (as S) | 0.003% |
Water | 0.03% |
RT | 22030 | 450 ml | |
RT | 22032 | 4x450 ml |
(Basic blue; Methylene Blue T50 or T Extra)
C15H16N3SCl FW 305.83 CAS #92-31-9
Dye Content: approx. 85% min.
Solubility: 3.25% Water; 1.75% Alcohol; 3.5% Cellosolve; 5.5% Glycol; 0.0% Xylene
*-A general stain for epoxy thick sections. Barajas et al.(1981) Identification of renal neuroeffector junctions by electron microscopy of reembedded light microscopic autoradiograms of semithin sections. J. Ultrastruct. Res. 77: 379.Campbell, R.D., And Hermans, C.O. (1972). A rapid method for resectioning 0.5 4.0 micron epoxy sections for electron microscopy. Stain Technol. 47:115.
RT | 22050 | 50g |
A Toluidine Blue/Basic Fuchsin Stain for GMA Embedded Tissue
(Tritolyl Phosphate)
(CH3C6H4O)3PO F.W. 368.37 CAS #1330-78-5
Specific Gravity (H2O=1): 1.16
A mixture of isomeric Tritolyl Phosphates, usually excluding the very toxic group ortho-isomer. Used to prepare benzoyl peroxide paste, which acts as a catalyst for water miscible resins.
RT | 22150 | 225 ml |
Please see DMP-30.
RT | 13600 | 25 ml |
Please see Picric Acid.
RT | 19550 | 100g |
White crystalline powder
C4H11NO3 F.W. 121.14 CAS #77-86-1
A 40% (w/v) aqueous solution. It is clear and colorless.
Assay >99.9%
Water .<0.2%
Heavy Metals.-2ppm
RT | 11700 | Tris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade | 100g |
RT | 11720 | Tris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade | 500g |
Please see listing in Prepared Buffers
RT | 11730 | 250 ml | |
RT | 11740 | 500 ml |
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
4-8°C | 64142-11 | Tris Buffer pH 10.0 | 100 ml |
4-8°C | 64142-12 | Tris Buffer pH 10.0 | 1000 ml |
(Polyethylene glycol tert-octylphenyl ether)
CAS #9002-93-1
Specific Gravity (H2O=1): 1.065
RT | 22140 | 100 ml | |
RT | 22142 | 500 ml |
Prepared 1% Triton X-100 in Deionized-Distilled water. Used for whole-cell preparations. Marek, L.F., and Kelly, R.O. (1983). A simple technique for the visualization of whole mount cytoskeletons with transmission electron microscopy. Anat. Rec. 207, 365. Pudney, J., and Singer, R.H. (1980). Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with Triton X-100. Tissue cell 12, 595.
RT | 22145 | 500 ml |
A prepared solution for the cleaning of diamond knives.
RT | 22146 | 500 ml |
CAS #9002-07-7
Extract from Porcine Pancreas.
Activity >100 NF units/mg
Used for whole cell preparations. (see references in Triton X-100)
RT | 22200 | 25g |
Description: Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes:
Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml
Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml
2-8°C | 64142-01 | Trypsin Kit Small | kit |
2-8°C | 64142-02 | Trypsin Kit Large | kit |
(Silicotungstic acid)
H4[SiO4.(W3O9)4]·xH2O M.W. 2878.29+aq CAS #11130-20-4
RT | 22240 | 25g |
Ingredients: NaCl; KCl; CaCl2.6H2O; MgCl2.6H2O; NaHCO3; NaH2PO4; Glucose; and Distilled water.
RT | 11760-05 | Tyrode Solution | 500 ml |
RT | 11760-10 | Tyrode Solution | 1 L |
UO2(OCOCH3)2·2H2O F.W. 424.14
CAS #541-09-3
Assay 98.0-102.0%
Specifications:
Insoluble Matters | 0.01 |
Chloride | 0.003% |
Sulfate | 0.01% |
Alkalies (as SO4) | 0.05% |
Heavy Metals | 0.002% |
Iron | 0.001% |
A universal EM stain for thin sections, en-block staining, and negative staining.
RT | 22400 | 25g |
Substitute for Uranyl Acetate
EMS is proud to introduce UranyLess, a new contrast stain solution for TEM, for all of your negative staining applications. It is an amazing substitute for Uranyl Acetate with similar results.
After only a minute of contact, UranyLess' fast-acting, non-radioactive lanthanide mix is finished staining your sections or deposits (see protocols below). If needed, lead citrate is recommended to increase the contrast. TIP: To prevent precipitates, use room temperature distilled water.
UranyLess's pH level is about 6.8-7. The 30ml airless bottle will stain approximately 1500 grids. The airless bottle increases shelf life, eliminates CO2 contamination, and produces less waste - the solution pumps out in perfect amounts without leaking or spilling. UranyLess is also available in a larger amount for use in automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.
UranyLess has been tested on many biological tissue (animal and plant): intestine, skeletal and cardiac muscle, liver, kidney, adrenal gland, nerve, cell culture, plant tissue, and also on negative staining of bacteriophage, bacteria, and polymers. UranyLess is ideal because of its ability to stain any kind of material and results are reproducible. To see the effectiveness of Uranyless, please visit the UranyLess Image Gallery.
UranyLess has a strong contrasting power, however, we recommend a Lead Citrate counterstain to enhance the contrast. You can follow the protocol of the UranyLess / Lead Citrate double contrast by watching the video. EMS recommends Lead Citrate 3%, ready to use. The special pump delivers the product without letting in air, thus preserving the solution and preventing CO2 dissolution. It is convenient to use because it performs in any position, even upside down. Comes in either a 30 ml Airless bottle or a 30ml Airless Syringe. The Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100
"Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules." Benmeradi N1,2, Payre B2 and Goodman SL3.
"C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle." Nature Communications. Published 23 Apr 2015. Sandrine Floriot, all.
RT | 22409 | UranyLess EM Stain* | ![]() |
30 ml |
RT | 22409-20 | UranyLess EM Stain | 200 ml | |
RT | 22410 | Lead Citrate 3%, Ready to use* | ![]() |
30 ml |
RT | 22410-01 | Lead Citrate Reynolds Stain 3%, Ready to use syringe** | 30 ml |
* in airless bottle
** Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100
A non-radioactive substitute for uranyl acetate with comparable results. Avoids the tedious authorization, shipping and disposal issues involving uranyl acetate without sacrificing performance. Two lanthanide salts, samarium and gadolinium triacetate, effectively used for staining plastic-embedded animal and plant tissues. Also for negative staining of macromolecules. Not recommended as a fixative.
RT | 22405 | UAR-EMS Stain | 25 ml |
IBI Blue Platinum Stain Pt4N8H6O24C20
An EM stain that may have the ability to replace Uranyl Acetate in some instances. If you have issues with having Uranium salts that are radioactive (depleted or not) in the lab this may be the answer for you. An alternative for staining TEM thin sections
RT | 22407 | Platinum Blue | 1 ml | 250.00 | Add to Cart |
We are now offering Uranyl Acetate pre-made and ready to use solution for making your work easier. The following percentages are avaialble off the shelf (1, 2, 3, 4). Other percentages are avaialble upon request.
RT | 22400-1 | 1% Uranyl Acetate Solution | ![]() |
100 ml |
RT | 22400-2 | 2% Uranyl Acetate Solution | ![]() |
100 ml |
RT | 22400-3 | 3% Uranyl Acetate Solution | ![]() |
100 ml |
RT | 22400-4 | 4% Uranyl Acetate Solution | ![]() |
100 ml |
UO2(HCO2)2·H2O
F.W. 378.08
CAS #16984-59-1
For ultra fine grain structure, our Uranyl Formate offers amazing advancement in negative staining for microscopy.
Purity | 99.9-100% |
Specific Gravity | 3.695 |
Appearance | Yellow Crystals |
Melting Point | 110°C |
RT | 22450 | Uranyl Formate | 1 gram |
RT | 22451 | Uranyl Formate | 5 grams |
UO2Mg(CH3COO)4
F.W.530.0
CAS # 20596-93-4
Used as a substitute for uranyl acetate in either double-staining techniques and also as a solo stain.
RT | 22500 | Uranyl Magnesium Acetate | 5 grams |
RT | 22501 | Uranyl Magnesium Acetate | 1 gram |
Reagent Grade, A.C.S.
UO2(NO3)2·6H2O
F.W. 502.13
CAS #13520-83-7
Purity | 99.9-100% |
Density | 2.807 g/cm3 |
Solubility | ~66g/100g H2O |
Melting Point | 60°C |
Boiling Point | 118°C |
Insoluble Matter | <0.005% |
Chloride (Cl) | <0.002% |
Sulfate (SO4) | <0.005% |
Alkalies % Alkaline Earths (Sulfates) | <0.1% |
Heavy Metals (as Pb) | <0.002% |
Iron (Fe) | <0.002% |
Substances Reducing Permanganate (as UIV) | <0.06% |
A Universal EM Negative Stain used for viruses. In tissue samples it stabilizes nucleic acids and cell membranes. These solutions are more stable than uranyl acetate and they react primarily with negatively charged groups in the absence of phosphate ions.
RT | 22600 | Uranyl Nitrate | 5 grams |
(COO)2 UO2 ·.3H2O
M.W.412.2
To adjust the pH of Uranyl compounds as well as a negative stain for electron microscopy.
RT | 22510 | Uranyl Oxalate | 1 gram |
ZnUO2(CH3COO)4
CAS # 10138-94-0
F.W. 571.59
Used as a laboratory reagent in the determination of sodium concentrations of solutions.
RT | 22610 | Uranyl Zinc Acetate | 5 grams |
Avoiding Contamination of Ultra-Thin Sections
Removal of Lead Citrate and Uranyl Acetate Precipitates on LR White Sections
To use with Weigert's Iodine or Weigert's Iron as a connective tissue stain.
RT | 26046-05 | Van Gieson's Solution | 500 ml |
RT | 26046-06 | Van Gieson's Solution | 1 L |
H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
Coliform negative
RT | 22800-01 | Deionized Water | 1 gal |
RT | 22800-05 | Deionized Water | 5 gal |
A histological stain. Demonstration of connective tissue is useful in cases of emphysema, arteriosclerosis and other vascular diseases
RT | 26045-05 | Weigert's Iodine Solution | 500 ml |
RT | 26045-06 | Weigert's Iodine Solution | 1 L |
Stain selectivity for nuclei, fungal, and general applications. Ready-to-use.
RT | 26044-05 | Weigert's Iron Hematoxylin A | 500 ml |
RT | 26044-06 | Weigert's Iron Hematoxylin A | 1 L |
RT | 26044-15 | Weigert's Iron Hematoxylin B | 500 ml |
RT | 26044-16 | Weigert's Iron Hematoxylin B | 1 L |
CAS #68988-92-1
Solubility: 0.091% Water; 0.23% Alcohol
RT | 22720 | 25 g |
For use as a blood stain. Ready-to-use.
RT | 26060-05 | Wright Stain Solution | 500 ml |
RT | 26060-06 | Wright Stain Solution | 1 L |
(Xylol, Dimethylbenzene)
C6H4(CH3)2 FW 106.27 CAS #1330-20-7
Specific Gravity (H2O=1): 0.865
b.p. 136-140°C
This reagent is a mixture of isomers (ortho, meta, and para) and may contain Ethylbenzene.
Color (A.P.H.A.) | 10 |
Subs. darkened by H2SO4 | To pass test |
Sulfur Compounds (as S) | 0.003% |
Water | 0.05% |
Residue after evaporation | 0.002% |
RT | 23400 | 450 ml |
RT | 23402 | Xylene:Peanut oil, 2:1 | 500 ml |
A xylene substitute for use in histology and cytology. It is safer and a more preferable alternative to xylene. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives.
It can be used in all procedures that require xylene. Biodegradable.
RT | 23410-01 | Xylene Substitute | 1 gal |
RT | 23410-04 | Xylene Substitute | 4x1 gal |
RT | 23410-05 | Xylene Substitute | 5 gal |
The safest choice for a xylene alternative available today
Reduce your risks & enhance your personnel safety with "The Original" high quality performance clearing agent. It performs equal-to or better than Xylene, yet smells like water! A non-hazardous clearing agent, it is non-flammable and odorless, with no vapor disguising additives.
RT | 23401-01 | SlideBrite™ Xylene Substitute | 1 gal |
RT | 23401-04 | SlideBrite™ Xylene Substitute | 4 x 1 gal |
Histosol™ is the original high flash point (114°F TCC) histological clearing agent. It is intended to be used as a replacement for xylene where the hazards associated with aromatic hydrocarbon vapors are to be reduced. Museum-quality tissue slides can be prepared with Hitosol without change in protocol or procedure. Histosol is manufactured from petrochemical products and is miscible in all proportions with ethanol, isopropanol, and t-butanol. It is also miscible with all paraffin-based tissue embedding media and all permanent mounting materials.
RT | 23405-01 | Histosol™ Xylene Substitute | 1 gal |
RT | 23405-04 | Histosol™ Xylene Substitute | 4 x 1 gal |
An intensive study from 1992 throughout 2000 has shown that Hemo-De® (d-Limonene) a terpene-based chemical with a pleasant citrus fragrance, was able to replace many of the clinical laboratories toxic reagents, such as carbol-xylene, xylene, ethyl acetate and formalin.
In Parasitology – Hemo-De replaces ethyl acetate in the concentration procedure; Hemo-De replaces carbol-xylene, xylene and formalin in the trichrome procedure.
In Histology – Hemo-De replaces xylene in most all procedures. Hem-De is a superior solvent and Clearing agent that can be used in all tissue processing, deparaffiniating and slide preparation.
In Research and Medical – Hematology, Cytology, Pathology, Microbiology, Histology, Pathology, Hospital
In Mechanical Applications – Cleaning of machine parts, cleaning tools, engines etc.
In Electronic Applications – Circuits boards, electronic components, tools.
General Applications – Clean metal and glass surfaces. Dilute with water can be used to clean all plastic surfaces. Removal of adhesives, glues, tars…
Chemical name: | d-Limonene (Terpene Hydrocarbon, n.o.s.) |
Composition: | d-Limonene, CAS [5989-27-5]98.0% Butylated Hydroxyanisolve (BHA), CAS [25013-16-5]…….<0.024% |
Flash Point (TCC): | 121°F (49.4°C) |
Boiling Point: | 349°F (176°C) @ 760mmHg |
Freezing Point: | -74°C |
Shipping: | Terpene Hydrocarbons, n.o.s. ID No. 04858003, Flammable Liquid, Group III |
RT | 23412-01 | Xylene Substitute – Hemo-De® | 1 gal |
RT | 23412-04 | Xylene Substitute – Hemo-De® | 4 x 1 gal |
RT | 23412-05 | Xylene Substitute – Hemo-De® | 5 gal |
*Hemo-De® is a product of Scientific Solvents.
Histo-Clear® (Distilled essential oils – food grade) and Histo-Clear II® (A mixture of Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.
RT | 64110-01 | Histo-Clear® | 1 L |
RT | 64110-04 | Histo-Clear® | 1 gal |
RT | 64110-10 | Histo-Clear® | 5 gal |
RT | 64111-01 | Histo-Clear II® | 1 L |
RT | 64111-04 | Histo-Clear II® | 1 gal |
RT | 64111-10 | Histo-Clear II® | 5 gal |
Concentrate for buffered aqueous zinc formalin
Zinc formalin fixatives improve general morphology and immunohistochemical staining and can reduce or eliminate the need for antigen recovery procedures while preserving immunoreactivity for months.
RT | 64125-01 | Z-Fix Concentrate, 1 gal | each |
This unique primer affords you a better adhesion between your resin and specimen that you are embedding and prevents the sample from pulling away from the resin during sectioning.
RT | 50440-10 | Z6040 Primer | 100 ml |
Наименование | Объем | Производство | Метод | Кат.Номер |
---|---|---|---|---|
Trypsin 1:100, Powder | 25 g | Electron Microscopy Sciences | 22200 ![]() |
|
Реактив 2,4,6-Tri (Dimethylaminomethyl)-Phenol | 25 мл | Electron Microscopy Sciences | 13600 ![]() |
|
Тритон X-100, 100 мл | 100 мл | Electron Microscopy Sciences | 22140 ![]() |
|
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