ООО «Лабораторная Диагностика»
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Каталог продукции
  Клеточные технологии и
  Стволовые клетки (Stem Cells)  
  Общая информация
  Выделение стволовых клеток:
  · Сортер клеток MoFlo  
  · Опыт применения  
  Тестирование стволовых клеток:
  · Бак. Анализ на стерильность  
  · Вирусная и бактериальная диагностика  
  · HLA - генотипирование  
  · Проточная цитометрия  
  · Типирование эритроцитов пуповинной     крови (cord blood) 
  · Флуоресцентная микроскопия  
  Реагенты для культивирования,   анализа и дифференцировки   стволовых клеток:
  · Производства Dako  
  · Рекомбинантные белки (Prospec) 
  Оборудование для культивирования   стволовых клеток:
  · Роботизированный комплекс HAMILTON 
  · Innovatis Cellscreen 
  Иммуноферментный анализ (ИФА)
  Клеточные технологии  
  Клиническая биохимия
  Мультиплексный анализ  
  Оборудование для биохимии и
  молекулярной биологии
  Общелабораторное оборудование
  Пищевая промышленность  
  Программное обеспечение  
  Проточная цитометрия
  Рекомбинантные белки
  Репродуктивные технологии
  Сортировка клеток
  Цитогенетическая диагностика


Flow Cytometry References

Researchers around the world and across many scientific disciplines rely on the MoFlo® High-Performance Cell Sorter. A partial list of journal articles highlighting some of their cutting-edge science follows.

Stem Cells

Akashi, K., X. He, J. Chen, H. Iwasaki, C. Niu, B. Steenhard, J. Zhang, J. Haug, and L. Li. 2003. Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis. Blood 101:383.


Asakura, A., P. Seale, A. Girgis-Gabardo, and M. A. Rudnicki. 2002. Myogenic specification of side population cells in skeletal muscle. J. Cell Biol. 159:123.


Bannert, N., M. Farzan, D. S. Friend, H. Ochi, K. S. Price, J. Sodroski, and J. A. Boyce. 2001. Human mast cell progenitors can be infected by macrophagetropic human immunodeficiency virus type 1 and retain virus with maturation in vitro. J Virol 75:10808.


Batten, M., J. Groom, T. G. Cachero, F. Qian, P. Schneider, J. Tschopp, J. L. Browning, and F. Mackay. 2000. BAFF mediates survival of peripheral immature B lymphocytes. J Exp Med 192:1453.


Burchert, A., S. Wolfl, M. Schmidt, C. Brendel, B. Denecke, D. Cai, L. Odyvanova, T. Lahaye, M. C. Muller, T. Berg, H. Gschaidmeier, B. Wittig, R. Hehlmann, A. Hochhaus, and A. Neubauer. 2003. Interferon-a, but not the ABL-kinase inhibitor imatinib (STI571), induces expression of myeloblastin and a specific T-cell response in chronic myeloid leukemia. Blood 101:259.


Chatterjee, S., W. Li, C. A. Wong, G. Fisher-Adams, D. Lu, M. Guha, J. A. Macer, S. J. Forman, and K. K. Wong, Jr. 1999. Transduction of primitive human marrow and cord blood-derived hematopoietic progenitor cells with adeno-associated virus vectors. Blood 93:1882.


Clarke, S. H., and L. W. Arnold. 1998. B-1 cell development: evidence for an uncommitted immunoglobulin (Ig)M+ B cell precursor in B-1 cell differentiation. J Exp Med 187:1325.


Dahl, A. M., C. Klein, P. G. Andres, C. A. London, M. P. Lodge, R. C. Mulligan, and A. K. Abbas. 2000. Expression of Bcl-XL restores cell survival, but not proliferation and effector differentiation, in CD28-deficient T lymphocytes. J. Exp. Med. 191:2031.


Fleming, H. E., and C. J. Paige. 2001. Pre-B cell receptor signaling mediates selective response to IL-7 at the pro-B to pre-B cell transition via an ERK/MAP kinase-dependent pathway. Immunity 15:521.


Ghia, P., P. Transidico, J. P. Veiga, C. Schaniel, F. Sallusto, K. Matsushima, S. E. Sallan, A. G. Rolink, A. Mantovani, L. M. Nadler, and A. A. Cardoso. 2001. Chemoattractants MDC and TARC are secreted by malignant B-cell precursors following CD40 ligation and support the migration of leukemia-specific T cells. Blood 98:533.


Goan, S. R., I. Junghahn, M. Wissler, M. Becker, J. Aumann, U. Just, G. Martiny-Baron, I. Fichtner, and R. Henschler. 2000. Donor stromal cells from human blood engraft in NOD/SCID mice. Blood 96:3971.


Gongora, R., R. P. Stephan, Z. Zhang, and M. D. Cooper. 2001. An essential role for Daxx in the inhibition of B lymphopoiesis by type I interferons. Immunity 14:727.


Habibian, H. K., S. O. Peters, C. C. Hsieh, J. Wuu, K. Vergilis, C. I. Grimaldi, J. Reilly, J. E. Carlson, A. E. Frimberger, F. M. Stewart, and P. J. Quesenberry. 1998. The fluctuating phenotype of the lymphohematopoietic stem cell with cell cycle transit. J Exp Med 188:393.


Henckaerts, E., H. Geiger, J. C. Langer, P. Rebollo, G. Van Zant, and H. W. Snoeck. 2002. Genetically determined variation in the number of phenotypically defined hematopoietic progenitor and stem cells and in their response to early-acting cytokines. Blood 99:3947.


Herblot, S., P. D. Aplan, and T. Hoang. 2002. Gradient of E2A activity in B-cell development. Mol Cell Biol 22:886.


Izon, D. J., J. C. Aster, Y. He, A. Weng, F. G. Karnell, V. Patriub, L. Xu, S. Bakkour, C. Rodriguez, D. Allman, and W. S. Pear. 2002. Deltex1 redirects lymphoid progenitors to the B cell lineage by antagonizing Notch1. Immunity 16:231.


Jackson, K. A., T. Mi, and M. A. Goodell. 1999. Hematopoietic potential of stem cells isolated from murine skeletal muscle. PNAS U S A 96:14482.


Jackson, K. A., S. M. Majka, H. Wang, J. Pocius, C. J. Hartley, M. W. Majesky, M. L. Entman, L. H. Michael, K. K. Hirschi, and M. A. Goodell. 2001. Regeneration of ischemic cardiac muscle and vascular endothelium by adult stem cells. J Clin Invest 107:1395.


Kaeser, P. S., M. A. Klein, P. Schwarz, and A. Aguzzi. 2001. Efficient lymphoreticular prion propagation requires PrP(c) in stromal and hematopoietic cells. J Virol 75:7097.


Kirby, S., W. Walton, and O. Smithies. 2000. Hematopoietic stem cells with controllable tEpoR transgenes have a competitive advantage in bone marrow transplantation. Blood 95:3710.


Kouro, T., K. L. Medina, K. Oritani, and P. W. Kincade. 2001. Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators. Blood 97:2708.


Kouro, T., V. Kumar, and P. W. Kincade. 2002. Relationships between early B- and NK-lineage lymphocyte precursors in bone marrow. Blood 100:3672.


Kubota, H., and L. M. Reid. 2000. Clonogenic hepatoblasts, common precursors for hepatocytic and biliary lineages, are lacking classical major histocompatibility complex class I antigen. PNAS  97:12132.


Kuehnle, I., M. H. Huls, Z. Liu, M. Semmelmann, R. A. Krance, M. K. Brenner, C. M. Rooney, and H. E. Heslop. 2000. CD20 monoclonal antibody (rituximab) for therapy of Epstein-Barr virus lymphoma after hematopoietic stem-cell transplantation. Blood 95:1502.


Lu, L. S., J. Tung, N. Baumgarth, O. Herman, M. Gleimer, and L. A. Herzenberg. 2002. Identification of a germ-line pro-B cell subset that distinguishes the fetal/neonatal from the adult B cell development pathway. PNAS 99:3007.


Majka, S. M., K. A. Jackson, K. A. Kienstra, M. W. Majesky, M. A. Goodell, and K. K. Hirschi. 2003. Distinct progenitor populations in skeletal muscle are bone marrow derived and exhibit different cell fates during vascular regeneration. J Clin Invest 111:71.


Manz, M. G., T. Miyamoto, K. Akashi, and I. L. Weissman. 2002. Prospective isolation of human clonogenic common myeloid progenitors. PNAS 99:11872.


Martin, F., and J. F. Kearney. 2000. Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and btk. Immunity 12:39.


McKinney-Freeman, S. L., K. A. Jackson, F. D. Camargo, G. Ferrari, F. Mavilio, and M. A. Goodell. 2002. Muscle-derived hematopoietic stem cells are hematopoietic in origin. PNAS 99:1341.


Metcalf, D., L. Di Rago, and S. Mifsud. 2002. Synergistic and inhibitory interactions in the in vitro control of murine megakaryocyte colony formation. Stem Cells 20:552.


Mikkola, H. K. A., Y. Fujiwara, T. M. Schlaeger, D. Traver, and S. H. Orkin. 2003. Expression of CD41 marks the initiation of definitive hematopoiesis in the mouse embryo. Blood 101:508.


Moore, K. A., H. Ema, and I. R. Lemischka. 1997. In vitro maintenance of highly purified, transplantable hematopoietic stem cells. Blood 89:4337.


Nilsson, S. K., M. S. Dooner, and P. J. Quesenberry. 1997. Synchronized cell-cycle induction of engrafting long-term repopulating stem cells. Blood 90:4646.


Ogilvy, S., D. Metcalf, L. Gibson, M. L. Bath, A. W. Harris, and J. M. Adams. 1999. Promoter elements of vav drive transgene expression in vivo throughout the hematopoietic compartment. Blood 94:1855.


Okuno, Y., H. Iwasaki, C. S. Huettner, H. S. Radomska, D. A. Gonzalez, D. G. Tenen, and K. Akashi. 2002. Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. PNAS 99:6246.


Okuno, Y., C. S. Huettner, H. S. Radomska, V. Petkova, H. Iwasaki, K. Akashi, and D. G. Tenen. 2002. Distal elements are critical for human CD34 expression in vivo. Blood 100:4420.


Pyatt, D. W., W. S. Stillman, Y. Yang, S. Gross, J. H. Zheng, and R. D. Irons. 1999. An essential role for NF-kB in human CD34+ bone marrow cell survival. Blood 93:3302.


Radomska, H. S., D. A. Gonzalez, Y. Okuno, H. Iwasaki, A. Nagy, K. Akashi, D. G. Tenen, and C. S. Huettner. 2002. Transgenic targeting with regulatory elements of the human CD34 gene. Blood 100:4410.


Reddy, G. P., C. Y. Tiarks, L. Pang, J. Wuu, C. C. Hsieh, and P. J. Quesenberry. 1997. Cell cycle analysis and synchronization of pluripotent hematopoietic progenitor stem cells. Blood 90:2293.


Santulli-Marotto, S., M. W. Retter, R. Gee, M. J. Mamula, and S. H. Clarke. 1998. Autoreactive B cell regulation: peripheral induction of developmental arrest by lupus-associated autoantigens. Immunity 8:209.


Schaniel, C., L. Bruno, F. Melchers, and A. G. Rolink. 2002. Multiple hematopoietic cell lineages develop in vivo from transplanted Pax5-deficient pre-B I-cell clones. Blood 99:472.


Shih, C. C., M. C. Hu, J. Hu, J. Medeiros, and S. J. Forman. 1999. Long-term ex vivo maintenance and expansion of transplantable human hematopoietic stem cells. Blood 94:1623.


Shih, C. C., M. C. Hu, J. Hu, Y. Weng, P. J. Yazaki, J. Medeiros, and S. J. Forman. 2000. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood 95:1957.


Shih, C. C., Y. Weng, A. Mamelak, T. LeBon, M. C. Hu, and S. J. Forman. 2001. Identification of a candidate human neurohematopoietic stem-cell population. Blood 98:2412.


Spyridonidis, A., M. Schmidt, W. Bernhardt, A. Papadimitriou, M. Azemar, W. Wels, B. Groner, and R. Henschler. 1998. Purging of mammary carcinoma cells during ex vivo culture of CD34+ hematopoietic progenitor cells with recombinant immunotoxins. Blood 91:1820.


Stewart, F. M., S. Zhong, J. Wuu, C. Hsieh, S. K. Nilsson, and P. J. Quesenberry. 1998. Lymphohematopoietic engraftment in minimally myeloablated hosts. Blood 91:3681.


Wright, D. E., E. P. Bowman, A. J. Wagers, E. C. Butcher, and I. L. Weissman. 2002. Hematopoietic stem cells are uniquely selective in their migratory response to chemokines. J Exp Med 195:1145.


Wulf, G. G., R. Y. Wang, I. Kuehnle, D. Weidner, F. Marini, M. K. Brenner, M. Andreeff, and M. A. Goodell. 2001. A leukemic stem cell with intrinsic drug efflux capacity in acute myeloid leukemia. Blood 98:1166.


Zhong, J. F., Y. Zhan, W. F. Anderson, and Y. Zhao. 2002. Murine hematopoietic stem cell distribution and proliferation in ablated and nonablated bone marrow transplantation. Blood 100:3521.

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info@LD.ru   тел.: +7 495 369-20-43