ООО «Лабораторная Диагностика»
ООО «Лабораторная Диагностика» info@LD.ru    тел.: +7 495 369-20-43
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ImmunoGold реагенты

arrow12Goat-anti-Rabbit IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25100 1.5 25101 .6
4-8°C EM-grade 2nm 25105 1.0 25106 .4
4-8°C EM-grade 6nm 25103 2.5 25104 1.0
4-8°C EM-grade 10nm 25108 2.5 25109 1.0
4-8°C EM-grade 15nm 25112 3.5 25113 1.4
4-8°C EM-grade 25nm 25115 3.5 25116 1.4

msdsarrow12Goat-anti-Mouse IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25120 1.5 25121 .6
4-8°C EM-grade 2nm 25125 1.0 25126 .4
4-8°C EM-grade 6nm 25123 2.5 25124 1.0
4-8°C EM-grade 10nm 25128 2.5 25129 1.0
4-8°C EM-grade 15nm 25132 3.5 25133 1.4
4-8°C EM-grade 25nm 25135 3.5 25136 1.4

msdsarrow12Goat-anti-Mouse IgM (µ-chain)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25140 1.5 25141 .6
4-8°C EM-grade 6nm 25143 2.5 25144 1.0
4-8°C EM-grade 10nm 25148 2.5 25149 1.0
4-8°C EM-grade 15nm 25152 3.5 25153 1.4
4-8°C EM-grade 25nm 25155 3.5 25156 1.4

msdsarrow12Goat-anti-Mouse IgG+IgM

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25160 1.5 25161 .6
4-8°C EM-grade 6nm 25163 2.5 25164 1.0
4-8°C EM-grade 10nm 25168 2.5 25169 1.0
4-8°C EM-grade 15nm 25172 3.5 25173 1.4
4-8°C EM-grade 25nm 25175 3.5 25176 1.4

msdsarrow12Goat-anti-Rat IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25180 1.5 25181 .6
4-8°C EM-grade 6nm 25183 2.5 25184 1.0
4-8°C EM-grade 10nm 25188 2.5 25189 1.0
4-8°C EM-grade 15nm 25192 3.5 25193 1.4
4-8°C EM-grade 25nm 25195 3.5 25196 1.4

msdsarrow12Goat-anti-Human IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25200 1.5 25201 .6
4-8°C EM-grade 6nm 25203 2.5 25204 1.0
4-8°C EM-grade 10nm 25208 2.5 25209 1.0
4-8°C EM-grade 15nm 25212 3.5 25213 1.4
4-8°C EM-grade 25nm 25215 3.5 25216 1.4

msdsarrow12Rabbit-anti-Goat IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25220 1.5 25221 .6
4-8°C EM-grade 6nm 25223 2.5 25224 1.0
4-8°C EM-grade 10nm 25228 2.5 25229 1.0
4-8°C EM-grade 15nm 25232 3.5 25233 1.4
4-8°C EM-grade 25nm 25235 3.5 25236 1.4

msdsarrow12Goat-anti-Biotin

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25240 1.5 25241 .6
4-8°C EM-grade 6nm 25243 2.5 25244 1.0
4-8°C EM-grade 10nm 25248 2.5 25249 1.0
4-8°C EM-grade 15nm 25252 3.5 25253 1.4
4-8°C EM-grade 25nm 25255 3.5 25256 1.4

msdsarrow12Streptavidin

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25260 1.5 25261 .6
4-8°C EM-grade 6nm 25263 2.5 25264 1.0
4-8°C EM-grade 10nm 25268 2.5 25269 1.0
4-8°C EM-grade 15nm 25272 3.5 25273 1.4
4-8°C EM-grade 25nm 25275 3.5 25276 1.4

msdsarrow12Protein A

  Particle Size Cat. # ml Cat. # ml
4-8°C EM-grade 6nm 25282 2.5 25283 1.0
4-8°C EM-grade 10nm 25284 2.5 25285 1.0
4-8°C EM-grade 15nm 25286 3.5 25287 1.4
4-8°C EM-grade 25nm 25288 3.5 25289 1.4

msdsarrow12Biotinylated Albumin

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25290 1.5 25291 .6
4-8°C EM-grade 10nm 25298 2.5 25299 1.0
4-8°C EM-grade 15nm 25302 3.5 25303 1.4
4-8°C EM-grade 25nm 25305 3.5 25306 1.4

msdsarrow12Mouse Monoclonal anti-FITC Gold Conjugates

For the electron microscopic visualization of FITC labels used in light microscopic specimens. For retrieval of faded FITC in DNA/RNA probes, fatty acids, and proteins.

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25580 1.5 25581 .6
4-8°C EM-grade 10nm 25582 2.5 25583 1.0

msdsarrow12Goat-anti-Chicken IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25584 1.5 25585 .6
4-8°C EM-grade 6nm 25586 2.5 25587 1.0
4-8°C EM-grade 10nm 25588 2.5 25589 1.0
4-8°C EM-grade 15nm 25590 3.5 25591 1.4
4-8°C EM-grade 25nm 25592 3.5 25593 1.4

msdsarrow12Protein G

  Particle Size Cat. # ml Cat. # ml
4-8°C EM-grade 6nm 25312 2.5 25313 1.0
4-8°C EM-grade 10nm 25314 2.5 25315 1.0
4-8°C EM-grade 15nm 25316 3.5 25317 1.4
4-8°C EM-grade 25nm 25318 3.5 25319 1.4

msdsarrow12Goat-anti-Guinea Pig IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25320 1.5 25321 .6
4-8°C EM-grade 6nm 25323 2.5 25324 1.0
4-8°C EM-grade 10nm 25328 2.5 25329 1.0
4-8°C EM-grade 15nm 25332 3.5 25333 1.4
4-8°C EM-grade 25nm 25335 3.5 25336 1.4

msdsarrow12Rabbit-anti-Sheep IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25340 1.5 25341 .6
4-8°C EM-grade 6nm 25343 2.5 25344 1.0
4-8°C EM-grade 10nm 25348 2.5 25349 1.0
4-8°C EM-grade 15nm 25352 3.5 25353 1.4
4-8°C EM-grade 25nm 25355 3.5 25356 1.4

msdsarrow12F(ab') 2 Fragment of Goat-anti-Rabbit IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25360 1.5 25361 .6
4-8°C EM-grade 6nm 25363 2.5 25364 1.0
4-8°C EM-grade 10nm 25362 2.5 25365 1.0
4-8°C EM-grade 15nm 25366 3.5 25367 1.4
4-8°C EM-grade 25nm 25368 3.5 25369 1.4

msdsarrow12F(ab') 2 Fragment of Goat-anti-Mouse IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25370 1.5 25371 .6
4-8°C EM-grade 6nm 25373 2.5 25374 1.0
4-8°C EM-grade 10nm 25372 2.5 25375 1.0
4-8°C EM-grade 15nm 25376 3.5 25377 1.4
4-8°C EM-grade 25nm 25378 3.5 25379 1.4

msdsarrow12F(ab') 2 Fragment of Goat-anti-Mouse IgG+IgM

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25380 1.5 25381 .6
4-8°C EM-grade 6nm 25383 2.5 25384 1.0
4-8°C EM-grade 10nm 25382 2.5 25385 1.0
4-8°C EM-grade 15nm 25386 3.5 25387 1.4
4-8°C EM-grade 25nm 25388 3.5 25389 1.4

msdsarrow12F(ab) Fragment of Sheep-anti-Digoxigenin

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25410 1.5 25411 .6
4-8°C EM-grade 10nm 25398 2.5 25399 1.0

msdsarrow12F(ab) Fragment of Goat-anti-Rabbit IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25412 1.5 25413 .6

msdsarrow12F(ab) Fragment of Goat anti-Mouse IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25414 1.5 25415 .6

msdsarrow12F(ab) Fragment of Goat-anti-Rat IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25416 1.5 25417 .6

msdsarrow12Rabbit-anti-Chicken IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25420 1.5 25421 .6
4-8°C EM-grade 6nm 25423 2.5 25424 1.0
4-8°C EM-grade 10nm 25428 2.5 25429 1.0
4-8°C EM-grade 15nm 25432 3.5 25433 1.4
4-8°C EM-grade 25nm 25435 3.5 25436 1.4

arrow12Cross Adsorbed Products

Cross reactivity can be a problem when working with primary antibodies sourced from animal species that are evolutionary closely related to the source of tissues or cells that antigens are to be detected in.

An example is using a mouse monoclonal on rat tissue. With many monoclonal antibodies of mouse origin and some of rat, and the prevailing animal cells and tissues being used in studies likewise originating from rat or mouse, it often happens that cross reactivity occurs, leading to false positive results.

A second problem that derives from cross-reactivity occurs in double labeling, when using primary antibodies from closely related animal sources. With this in mind, Aurion has developed two series of new conjugates:

Goat-anti-Mouse IgG (H+L)xRat (cross adsorbed against Rat immunoglobulins)
  Particle Size Cat. # ml Cat. # ml
4-8°C EM-grade Ultra Small 25900 1.5 25901 0.6
4-8°C EM-grade 10nm 25902 2.5 25903 1.0
Goat-anti-Rat IgG (H+L)xMouse (cross adsorbed against Mouse immunoglobulins)
  Particle Size Cat. # ml Cat. # ml
4-8°C EM-grade Ultra Small 25904 1.5 25905 0.6
4-8°C EM-grade 10nm 25906 2.5 25907 1.0

Secondary antibodies have been thoroughly purified using solid phase technique. These purified antibodies allow detection of antigens with mouse monoclonal antibodies in rat tissue or rat monoclonal antibodies in mouse tissue, without causing interfering binding to either endogenous mouse or rat immunoglobulins.

The antibodies can be used for double labeling as well when both mouse and rat primary antibodies are used with other than mouse or rat tissue.

Please note

Cross adsorption against closely related species may result in epitope recognition with reduced binding force and lower labeling density.

EMS and Aurion would like to inform you that the strongest labeling intensity is usually achieved with the least refined antibodies. Users are advised to take this into account when choosing a product from the Aurion range of anti-mouse or anti-rat conjugates. In case of doubt, our technical staff will gladly assist you in making the best choice.

arrow12Donkey Conjugate Line

In many cases double labeling experiments are based on using two primary antibodies from different animal species, for instance mouse and rabbit. Of late, we have noticed an increasing number of users that have primary antibodies developed in goat. It is impossible to perform a double labeling experiment in combination with, for instance a mouse primary antibody, since applicable secondary antibodies are either raised in rabbit or goat. Hence the Goat-anti-Mouse would be recognized by the Rabbit-anti-Goat, and it would be impossible to get reliable results. In the past, only using directly labeled primary antibodies would solve this. This would require researchers to go through the lengthy procedure of preparing such conjugates and even then with limited success.

To overcome this difficulty we are proud to release the Aurion Series of Secondary ImmunoGold conjugates based on antibodies raised in Donkey, including Donkey-anti-Goat. It is then known that mixing Donkey-anti-Goat and Donkey-anti-Mouse provides a successful solution to the above mentioned case.

msdsarrow12Donkey-anti-Rabbit IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25700 1.5 25701 .6
4-8°C EM-grade 6nm 25702 2.5 25703 1.0
4-8°C EM-grade 10nm 25704 2.5 25705 1.0
4-8°C EM-grade 15nm 25706 3.5 25707 1.4
4-8°C EM-grade 25nm 25708 3.5 25709 1.4

msdsarrow12Donkey-anti-Goat IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25800 1.5 25801 .6
4-8°C EM-grade 6nm 25802 2.5 25803 1.0
4-8°C EM-grade 10nm 25804 2.5 25805 1.0
4-8°C EM-grade 15nm 25806 3.5 25807 1.4
4-8°C EM-grade 25nm 25808 3.5 25809 1.4

msdsarrow12Donkey-anti-Mouse IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25810 1.5 25811 .6
4-8°C EM-grade 6nm 25812 2.5 25813 1.0
4-8°C EM-grade 10nm 25814 2.5 25815 1.0
4-8°C EM-grade 15nm 25816 3.5 25817 1.4
4-8°C EM-grade 25nm 25818 3.5 25819 1.4

msdsarrow12Donkey-anti-Sheep IgG (H&L)

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25820 1.5 25821 .6
4-8°C EM-grade 6nm 25822 2.5 25823 1.0
4-8°C EM-grade 10nm 25824 2.5 25825 1.0
4-8°C EM-grade 15nm 25826 3.5 25827 1.4
4-8°C EM-grade 25nm 25828 3.5 25829 1.4

msdsarrow12Anionic Gold Tracer

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25460 10 25461 5
4-8°C EM-grade 6nm 25463 10 25464 5
4-8°C EM-grade 10nm 25466 10 25467 5
4-8°C EM-grade 15nm 25469 10 25470 5
4-8°C EM-grade 25nm 25472 10 25473 5

msdsarrow12BSA Gold Tracer

  Particle Size Cat. # ml Cat. # ml
4-8°C UltraSmall 25480 10 25481 5
4-8°C EM-grade 6nm 25483 10 25484 5
4-8°C EM-grade 10nm 25486 10 25487 5
4-8°C EM-grade 15nm 25489 10 25490 5
4-8°C EM-grade 25nm 25492 10 25493 5

arrow12Aurion BSA-Fragment Stabilized

  Particle Size Cat. # ml
4-8°C UltraSmall Gold 25498 20

msdsarrow12Gold Nanoparticles – Gold Sols

  Particle Size Cat. # ml
4-8°C Ultrasmall 25509 20
4-8°C 6nm 25510 100
4-8°C 10nm 25512 100
4-8°C 15nm 25514 100
4-8°C 25nm 25516 100

arrow12Aurion Gold Nanoparticles – Carboxyl Functionalized

  Particle Size Cat. # ml
4-8°C 6nm 25600 4x5
4-8°C 10nm 25601 4x5
4-8°C 15nm 25602 4x5
4-8°C 25nm 25603 4x5

msdsarrow12Blocking Solutions

Particle Size Cat. # ml
Basic Blocking (For Protein A&G) 25595 30
Goat Gold Conjugates 25596 30
Rabbit Gold Conjugates 25597 30
Sheep Gold Conjugates 25598 30
Donkey Gold Conjugates 25599 30

arrow12IMMUNOGOLD REAGENT KITS - EM GRADE

Complete kits with the following characteristics

  • Two non-overlapping gold particle sizes
  • Coupled to: either Goat-anti-Rabbit and Goat-anti-Mouse IgG antibodies
  • Affinity purified and cross adsorbed antibodies
  • For use with rabbit and mouse monoclonal and polyclonal primary antibodies
  • Acetylated serum albumin with increased exposure of negative charges
  • Unparalleled low background levels

Benefits

  • Covering over 90% of all applications in EM
  • For single and double labeling in Electron Microscopy
4-8°C 25540 EM-kit GAR-6/GAM-10/BSA-c™
1ml Goat-anti-Rabbit 6nm
1ml Goat-anti-Mouse IgG 10nm
30 ml BSA-c™ background suppressing agent
kit
4-8°C 25544 EM-kit GAM-6/GAR-10/BSA-c™
1ml Goat-anti-Mouse IgG 6nm
1ml Goat-anti-Rabbit 10nm
30 ml BSA-c™ background suppressing agent
kit
4-8°C 25548 EM-kit PrA-6/PrA-10/BSA-c™
1ml Protein A 6nm
1ml Protein A 10nm
30 ml BSA-c™ background suppressing agent
kit
4-8°C 25540-05 Custom EM Kit
For customization of our EM Kits you get to choose the 2 vials of your EM Grade Reagent. Supplied with the kit as well as 30 ml of BSA-c™.
kit

Benefits of our US-Kit

For EM

  • For the most sensitive labeling in electron microscopy
  • For the penetration in ultra thin cryosections resulting in enhanced detectability and sensitivity
  • New possibilities for pre-embedding labeling
  • For the labeling of at least 330 on-grid-specimens

For LM

  • Premium reagents for penetration in hydrated sections (paraffin, cryostat) and in slightly fixed cells
  • Permanent, non-fading silver signal
  • Most sensitive reagents compatible with counterstaining
  • For the immunolabeling of 200 slides

For Immunoblotting

  • Highest sensitivity
  • Black to brown/black signal suited for low level detection where colored precipitates fail
  • For the immunogold labeling of 10-20 nitrocellulose immunoblot strips
4-8°C 25550-05 Custom Ultra Small Kit 
For customization of our Ultra Small Kits we now have available a make it yourself kit where as you get to choose the two vials of your Ultra Small Reagent. Also supplied with the kit is 30 ml BSA-c™ and 2x30 ml of R-Gent SE-LM.
kit
4-8°C 25550-06 Custom Ultra Small Kit 
For customization of our Ultra-Small Kits we now have available a make it yourself kit where you get to choose the two vials of your Ultra-Small Reagent. Also supplied with the kit is 30 ml BSA-c™ and 30 ml SE-EM..
kit
4-8°C 25550 US-kit GAR/GAM/BSA-c™/R-Gent kit
•0.6ml Goat-anti-Rabbit GP-Ultra Small 0.6ml •Goat-anti-Mouse IgG GP-Ultra-Small
•30 ml BSA-c™ background suppressing agent
•R-Gent Silver Enhancement reagent
kit
4-8°C 25552 US-kit GAR/GAM/BSA-c™/R-Gent SE-EM
• 0.6ml Goat-anti-Rabbit GP-Ultra-Small
• 0.6ml Goat-anti-Mouse IgG GP-Ultra-Small
• 30 ml BSA-c™ background suppressing agent
• SE-EM Silver Enhancement
kit
  25550-10 Ultra-Small Starter kit
to give yourself the opportunity to make yourself familair with our Ultra-Small Gold Conjugates - we have our starter kit. These kits are sufficient for approximately 50 specimens. • 100 µl Ultra-Small Conjugate
• 10 ml BSA-c™
• 2x2.5 ml of R-Gent SE-LM and a sample pack of R-Gent SE-EM
kit

arrow12Blocking Solutions, Background Suppression, R-Gent Silver Enhancement

Cat # Descripton Pack
25520 AURION R-Gent Silver Enhancement Kitmsds 2x30 ml
25522 AURION R-Gent Silver Enhancement Kitmsds 2x250 ml
25521 AURION R-Gent SE-EM Kit 30 ml
25521-90 AURION R-Gent SE-EM Kit 90 ml
25536 COL-AURION Total Protein Stainmsds 500 ml
25554 Bovine Serum Albumin 25 gr
25557 AURION BSA-C (acetylated BSA)msds 30 ml
25558 AURION BSA-C (acetylated BSA)msds 100 ml
25560 Cold Water Fish Skin Gelatin 40%msds 10 ml
25564 Tween-20 10 ml
25568 Normal Rabbit Serummsds 5 ml
25570 Normal Goat Serummsds 5 ml
25574 Gum Arabic 20 gr
25578 Normal Sheep Serummsds 5 ml
25572 Normal Donkey Serummsds 5 ml
25830 Enhancement Conditioning Solution 100 ml

Technical Tip

Reactivity of Protein A and Protein

Информация для заказа

Наименование ОбъемПроизводствоМетод Кат.Номер
EM GOAT/MOUSE IgG, 15 nm  Electron Microscopy Sciences  25133 
   
© ООО «Лабораторная Диагностика»
info@LD.ru   тел.: +7 495 369-20-43