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ImmunoGold реагенты

Goat-anti-Rabbit IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25100	1.5	25101	.6
4-8°C	EM-grade 2nm	25105	1.0	25106	.4
4-8°C	EM-grade 6nm	25103	2.5	25104	1.0
4-8°C	EM-grade 10nm	25108	2.5	25109	1.0
4-8°C	EM-grade 15nm	25112	3.5	25113	1.4
4-8°C	EM-grade 25nm	25115	3.5	25116	1.4

Goat-anti-Mouse IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25120	1.5	25121	.6
4-8°C	EM-grade 2nm	25125	1.0	25126	.4
4-8°C	EM-grade 6nm	25123	2.5	25124	1.0
4-8°C	EM-grade 10nm	25128	2.5	25129	1.0
4-8°C	EM-grade 15nm	25132	3.5	25133	1.4
4-8°C	EM-grade 25nm	25135	3.5	25136	1.4

Goat-anti-Mouse IgM (µ-chain)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25140	1.5	25141	.6
4-8°C	EM-grade 6nm	25143	2.5	25144	1.0
4-8°C	EM-grade 10nm	25148	2.5	25149	1.0
4-8°C	EM-grade 15nm	25152	3.5	25153	1.4
4-8°C	EM-grade 25nm	25155	3.5	25156	1.4

Goat-anti-Mouse IgG+IgM

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25160	1.5	25161	.6
4-8°C	EM-grade 6nm	25163	2.5	25164	1.0
4-8°C	EM-grade 10nm	25168	2.5	25169	1.0
4-8°C	EM-grade 15nm	25172	3.5	25173	1.4
4-8°C	EM-grade 25nm	25175	3.5	25176	1.4

Goat-anti-Rat IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25180	1.5	25181	.6
4-8°C	EM-grade 6nm	25183	2.5	25184	1.0
4-8°C	EM-grade 10nm	25188	2.5	25189	1.0
4-8°C	EM-grade 15nm	25192	3.5	25193	1.4
4-8°C	EM-grade 25nm	25195	3.5	25196	1.4

Goat-anti-Human IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25200	1.5	25201	.6
4-8°C	EM-grade 6nm	25203	2.5	25204	1.0
4-8°C	EM-grade 10nm	25208	2.5	25209	1.0
4-8°C	EM-grade 15nm	25212	3.5	25213	1.4
4-8°C	EM-grade 25nm	25215	3.5	25216	1.4

Rabbit-anti-Goat IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25220	1.5	25221	.6
4-8°C	EM-grade 6nm	25223	2.5	25224	1.0
4-8°C	EM-grade 10nm	25228	2.5	25229	1.0
4-8°C	EM-grade 15nm	25232	3.5	25233	1.4
4-8°C	EM-grade 25nm	25235	3.5	25236	1.4

Goat-anti-Biotin

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25240	1.5	25241	.6
4-8°C	EM-grade 6nm	25243	2.5	25244	1.0
4-8°C	EM-grade 10nm	25248	2.5	25249	1.0
4-8°C	EM-grade 15nm	25252	3.5	25253	1.4
4-8°C	EM-grade 25nm	25255	3.5	25256	1.4

Streptavidin

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25260	1.5	25261	.6
4-8°C	EM-grade 6nm	25263	2.5	25264	1.0
4-8°C	EM-grade 10nm	25268	2.5	25269	1.0
4-8°C	EM-grade 15nm	25272	3.5	25273	1.4
4-8°C	EM-grade 25nm	25275	3.5	25276	1.4

Protein A

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	EM-grade 6nm	25282	2.5	25283	1.0
4-8°C	EM-grade 10nm	25284	2.5	25285	1.0
4-8°C	EM-grade 15nm	25286	3.5	25287	1.4
4-8°C	EM-grade 25nm	25288	3.5	25289	1.4

Biotinylated Albumin

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25290	1.5	25291	.6
4-8°C	EM-grade 10nm	25298	2.5	25299	1.0
4-8°C	EM-grade 15nm	25302	3.5	25303	1.4
4-8°C	EM-grade 25nm	25305	3.5	25306	1.4

Mouse Monoclonal anti-FITC Gold Conjugates

For the electron microscopic visualization of FITC labels used in light microscopic specimens. For retrieval of faded FITC in DNA/RNA probes, fatty acids, and proteins.

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25580	1.5	25581	.6
4-8°C	EM-grade 10nm	25582	2.5	25583	1.0

Goat-anti-Chicken IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25584	1.5	25585	.6
4-8°C	EM-grade 6nm	25586	2.5	25587	1.0
4-8°C	EM-grade 10nm	25588	2.5	25589	1.0
4-8°C	EM-grade 15nm	25590	3.5	25591	1.4
4-8°C	EM-grade 25nm	25592	3.5	25593	1.4

Protein G

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	EM-grade 6nm	25312	2.5	25313	1.0
4-8°C	EM-grade 10nm	25314	2.5	25315	1.0
4-8°C	EM-grade 15nm	25316	3.5	25317	1.4
4-8°C	EM-grade 25nm	25318	3.5	25319	1.4

Goat-anti-Guinea Pig IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25320	1.5	25321	.6
4-8°C	EM-grade 6nm	25323	2.5	25324	1.0
4-8°C	EM-grade 10nm	25328	2.5	25329	1.0
4-8°C	EM-grade 15nm	25332	3.5	25333	1.4
4-8°C	EM-grade 25nm	25335	3.5	25336	1.4

Rabbit-anti-Sheep IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25340	1.5	25341	.6
4-8°C	EM-grade 6nm	25343	2.5	25344	1.0
4-8°C	EM-grade 10nm	25348	2.5	25349	1.0
4-8°C	EM-grade 15nm	25352	3.5	25353	1.4
4-8°C	EM-grade 25nm	25355	3.5	25356	1.4

F(ab') 2 Fragment of Goat-anti-Rabbit IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25360	1.5	25361	.6
4-8°C	EM-grade 6nm	25363	2.5	25364	1.0
4-8°C	EM-grade 10nm	25362	2.5	25365	1.0
4-8°C	EM-grade 15nm	25366	3.5	25367	1.4
4-8°C	EM-grade 25nm	25368	3.5	25369	1.4

F(ab') 2 Fragment of Goat-anti-Mouse IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25370	1.5	25371	.6
4-8°C	EM-grade 6nm	25373	2.5	25374	1.0
4-8°C	EM-grade 10nm	25372	2.5	25375	1.0
4-8°C	EM-grade 15nm	25376	3.5	25377	1.4
4-8°C	EM-grade 25nm	25378	3.5	25379	1.4

F(ab') 2 Fragment of Goat-anti-Mouse IgG+IgM

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25380	1.5	25381	.6
4-8°C	EM-grade 6nm	25383	2.5	25384	1.0
4-8°C	EM-grade 10nm	25382	2.5	25385	1.0
4-8°C	EM-grade 15nm	25386	3.5	25387	1.4
4-8°C	EM-grade 25nm	25388	3.5	25389	1.4

F(ab) Fragment of Sheep-anti-Digoxigenin

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25410	1.5	25411	.6
4-8°C	EM-grade 10nm	25398	2.5	25399	1.0

F(ab) Fragment of Goat-anti-Rabbit IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25412	1.5	25413	.6

F(ab) Fragment of Goat anti-Mouse IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25414	1.5	25415	.6

F(ab) Fragment of Goat-anti-Rat IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25416	1.5	25417	.6

Rabbit-anti-Chicken IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25420	1.5	25421	.6
4-8°C	EM-grade 6nm	25423	2.5	25424	1.0
4-8°C	EM-grade 10nm	25428	2.5	25429	1.0
4-8°C	EM-grade 15nm	25432	3.5	25433	1.4
4-8°C	EM-grade 25nm	25435	3.5	25436	1.4

Cross Adsorbed Products

Cross reactivity can be a problem when working with primary antibodies sourced from animal species that are evolutionary closely related to the source of tissues or cells that antigens are to be detected in.

An example is using a mouse monoclonal on rat tissue. With many monoclonal antibodies of mouse origin and some of rat, and the prevailing animal cells and tissues being used in studies likewise originating from rat or mouse, it often happens that cross reactivity occurs, leading to false positive results.

A second problem that derives from cross-reactivity occurs in double labeling, when using primary antibodies from closely related animal sources. With this in mind, Aurion has developed two series of new conjugates:

Goat-anti-Mouse IgG (H+L)xRat (cross adsorbed against Rat immunoglobulins)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	EM-grade Ultra Small	25900	1.5	25901	0.6
4-8°C	EM-grade 10nm	25902	2.5	25903	1.0

Goat-anti-Rat IgG (H+L)xMouse (cross adsorbed against Mouse immunoglobulins)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	EM-grade Ultra Small	25904	1.5	25905	0.6
4-8°C	EM-grade 10nm	25906	2.5	25907	1.0

Secondary antibodies have been thoroughly purified using solid phase technique. These purified antibodies allow detection of antigens with mouse monoclonal antibodies in rat tissue or rat monoclonal antibodies in mouse tissue, without causing interfering binding to either endogenous mouse or rat immunoglobulins.

The antibodies can be used for double labeling as well when both mouse and rat primary antibodies are used with other than mouse or rat tissue.

Please note

Cross adsorption against closely related species may result in epitope recognition with reduced binding force and lower labeling density.

EMS and Aurion would like to inform you that the strongest labeling intensity is usually achieved with the least refined antibodies. Users are advised to take this into account when choosing a product from the Aurion range of anti-mouse or anti-rat conjugates. In case of doubt, our technical staff will gladly assist you in making the best choice.

Donkey Conjugate Line

In many cases double labeling experiments are based on using two primary antibodies from different animal species, for instance mouse and rabbit. Of late, we have noticed an increasing number of users that have primary antibodies developed in goat. It is impossible to perform a double labeling experiment in combination with, for instance a mouse primary antibody, since applicable secondary antibodies are either raised in rabbit or goat. Hence the Goat-anti-Mouse would be recognized by the Rabbit-anti-Goat, and it would be impossible to get reliable results. In the past, only using directly labeled primary antibodies would solve this. This would require researchers to go through the lengthy procedure of preparing such conjugates and even then with limited success.

To overcome this difficulty we are proud to release the Aurion Series of Secondary ImmunoGold conjugates based on antibodies raised in Donkey, including Donkey-anti-Goat. It is then known that mixing Donkey-anti-Goat and Donkey-anti-Mouse provides a successful solution to the above mentioned case.

Donkey-anti-Rabbit IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25700	1.5	25701	.6
4-8°C	EM-grade 6nm	25702	2.5	25703	1.0
4-8°C	EM-grade 10nm	25704	2.5	25705	1.0
4-8°C	EM-grade 15nm	25706	3.5	25707	1.4
4-8°C	EM-grade 25nm	25708	3.5	25709	1.4

Donkey-anti-Goat IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25800	1.5	25801	.6
4-8°C	EM-grade 6nm	25802	2.5	25803	1.0
4-8°C	EM-grade 10nm	25804	2.5	25805	1.0
4-8°C	EM-grade 15nm	25806	3.5	25807	1.4
4-8°C	EM-grade 25nm	25808	3.5	25809	1.4

Donkey-anti-Mouse IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25810	1.5	25811	.6
4-8°C	EM-grade 6nm	25812	2.5	25813	1.0
4-8°C	EM-grade 10nm	25814	2.5	25815	1.0
4-8°C	EM-grade 15nm	25816	3.5	25817	1.4
4-8°C	EM-grade 25nm	25818	3.5	25819	1.4

Donkey-anti-Sheep IgG (H&L)

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25820	1.5	25821	.6
4-8°C	EM-grade 6nm	25822	2.5	25823	1.0
4-8°C	EM-grade 10nm	25824	2.5	25825	1.0
4-8°C	EM-grade 15nm	25826	3.5	25827	1.4
4-8°C	EM-grade 25nm	25828	3.5	25829	1.4

Anionic Gold Tracer

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25460	10	25461	5
4-8°C	EM-grade 6nm	25463	10	25464	5
4-8°C	EM-grade 10nm	25466	10	25467	5
4-8°C	EM-grade 15nm	25469	10	25470	5
4-8°C	EM-grade 25nm	25472	10	25473	5

BSA Gold Tracer

	Particle Size	Cat. #	ml	Cat. #	ml
4-8°C	UltraSmall	25480	10	25481	5
4-8°C	EM-grade 6nm	25483	10	25484	5
4-8°C	EM-grade 10nm	25486	10	25487	5
4-8°C	EM-grade 15nm	25489	10	25490	5
4-8°C	EM-grade 25nm	25492	10	25493	5

Aurion BSA-Fragment Stabilized

	Particle Size	Cat. #	ml
4-8°C	UltraSmall Gold	25498	20

Gold Nanoparticles – Gold Sols

	Particle Size	Cat. #	ml
4-8°C	Ultrasmall	25509	20
4-8°C	6nm	25510	100
4-8°C	10nm	25512	100
4-8°C	15nm	25514	100
4-8°C	25nm	25516	100

Aurion Gold Nanoparticles – Carboxyl Functionalized

	Particle Size	Cat. #	ml
4-8°C	6nm	25600	4x5
4-8°C	10nm	25601	4x5
4-8°C	15nm	25602	4x5
4-8°C	25nm	25603	4x5

Blocking Solutions

Particle Size	Cat. #	ml
Basic Blocking (For Protein A&G)	25595	30
Goat Gold Conjugates	25596	30
Rabbit Gold Conjugates	25597	30
Sheep Gold Conjugates	25598	30
Donkey Gold Conjugates	25599	30

IMMUNOGOLD REAGENT KITS - EM GRADE

Complete kits with the following characteristics

Two non-overlapping gold particle sizes
Coupled to: either Goat-anti-Rabbit and Goat-anti-Mouse IgG antibodies
Affinity purified and cross adsorbed antibodies
For use with rabbit and mouse monoclonal and polyclonal primary antibodies
Acetylated serum albumin with increased exposure of negative charges
Unparalleled low background levels

Benefits

Covering over 90% of all applications in EM
For single and double labeling in Electron Microscopy

4-8°C	25540	EM-kit GAR-6/GAM-10/BSA-c™ 1ml Goat-anti-Rabbit 6nm 1ml Goat-anti-Mouse IgG 10nm 30 ml BSA-c™ background suppressing agent	kit
4-8°C	25544	EM-kit GAM-6/GAR-10/BSA-c™ 1ml Goat-anti-Mouse IgG 6nm 1ml Goat-anti-Rabbit 10nm 30 ml BSA-c™ background suppressing agent	kit
4-8°C	25548	EM-kit PrA-6/PrA-10/BSA-c™ 1ml Protein A 6nm 1ml Protein A 10nm 30 ml BSA-c™ background suppressing agent	kit
4-8°C	25540-05	Custom EM Kit For customization of our EM Kits you get to choose the 2 vials of your EM Grade Reagent. Supplied with the kit as well as 30 ml of BSA-c™.	kit

Benefits of our US-Kit

For EM

For the most sensitive labeling in electron microscopy
For the penetration in ultra thin cryosections resulting in enhanced detectability and sensitivity
New possibilities for pre-embedding labeling
For the labeling of at least 330 on-grid-specimens

For LM

Premium reagents for penetration in hydrated sections (paraffin, cryostat) and in slightly fixed cells
Permanent, non-fading silver signal
Most sensitive reagents compatible with counterstaining
For the immunolabeling of 200 slides

For Immunoblotting

Highest sensitivity
Black to brown/black signal suited for low level detection where colored precipitates fail
For the immunogold labeling of 10-20 nitrocellulose immunoblot strips

4-8°C	25550-05	Custom Ultra Small Kit For customization of our Ultra Small Kits we now have available a make it yourself kit where as you get to choose the two vials of your Ultra Small Reagent. Also supplied with the kit is 30 ml BSA-c™ and 2x30 ml of R-Gent SE-LM.	kit
4-8°C	25550-06	Custom Ultra Small Kit For customization of our Ultra-Small Kits we now have available a make it yourself kit where you get to choose the two vials of your Ultra-Small Reagent. Also supplied with the kit is 30 ml BSA-c™ and 30 ml SE-EM..	kit
4-8°C	25550	US-kit GAR/GAM/BSA-c™/R-Gent kit •0.6ml Goat-anti-Rabbit GP-Ultra Small 0.6ml •Goat-anti-Mouse IgG GP-Ultra-Small •30 ml BSA-c™ background suppressing agent •R-Gent Silver Enhancement reagent	kit
4-8°C	25552	US-kit GAR/GAM/BSA-c™/R-Gent SE-EM • 0.6ml Goat-anti-Rabbit GP-Ultra-Small • 0.6ml Goat-anti-Mouse IgG GP-Ultra-Small • 30 ml BSA-c™ background suppressing agent • SE-EM Silver Enhancement	kit
	25550-10	Ultra-Small Starter kit to give yourself the opportunity to make yourself familair with our Ultra-Small Gold Conjugates - we have our starter kit. These kits are sufficient for approximately 50 specimens. • 100 µl Ultra-Small Conjugate • 10 ml BSA-c™ • 2x2.5 ml of R-Gent SE-LM and a sample pack of R-Gent SE-EM	kit

Blocking Solutions, Background Suppression, R-Gent Silver Enhancement

Cat #	Descripton	Pack
25520	AURION R-Gent Silver Enhancement Kit	2x30 ml
25522	AURION R-Gent Silver Enhancement Kit	2x250 ml
25521	AURION R-Gent SE-EM Kit	30 ml
25521-90	AURION R-Gent SE-EM Kit	90 ml
25536	COL-AURION Total Protein Stain	500 ml
25554	Bovine Serum Albumin	25 gr
25557	AURION BSA-C (acetylated BSA)	30 ml
25558	AURION BSA-C (acetylated BSA)	100 ml
25560	Cold Water Fish Skin Gelatin 40%	10 ml
25564	Tween-20	10 ml
25568	Normal Rabbit Serum	5 ml
25570	Normal Goat Serum	5 ml
25574	Gum Arabic	20 gr
25578	Normal Sheep Serum	5 ml
25572	Normal Donkey Serum	5 ml
25830	Enhancement Conditioning Solution	100 ml

Technical Tip

Reactivity of Protein A and Protein

Информация для заказа

Наименование	Объем	Производство	Метод	Кат.Номер
Антитела Goat-anti-Mouse IgG (H&L), 15 nm, 1.4 мл		Electron Microscopy Sciences		25133