| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse |
| Tested Application | ELISA, WB, IF, IHC,IF |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | Q96RL1 |
| Gene ID | |
| Calculated MW | 164 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Ubiquitin-binding protein(PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin(PubMed:19328070, Ref.36). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks(DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. |
| Immunogen | ubiquitin interaction motif containing 1 |
| Synonyms | BRCA1 A complex subunit RAP80, RAP80, Receptor associated protein 80, RXRIP110, UIMC1, X2HRIP110 |
| Recommended dilution | WB: 1:200-1:1000; IP: 1：200-1：2000；IHC: 1：20-1：200; IF: 1：10-1：100 |
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab09247(UIMC1 Antibody) at dilution of 1:50
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mouse colon tissue were subjected to SDS PAGE followed by western blot with FNab09247(UIMC1 antibody) at dilution of 1:300
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