| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, WB, IHC, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | Q12774 |
| Gene ID | |
| Calculated MW | 60 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Guanine nucleotide exchange factor which activates Rho GTPases(PubMed:15601624). Strongly activates RHOA(PubMed:15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1(By similarity). Involved in regulation of cell shape and actin cytoskeletal organization(PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia(PubMed:14662653). Required for SRC-induced podosome formation(By similarity). Involved in positive regulation of immature dendritic cell migration(By similarity). |
| Immunogen | Rho guanine nucleotide exchange factor(GEF) 5 |
| Synonyms | TIM |
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab00563(ARHGEF5 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
 |
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab00563(ARHGEF5 antibody) at dilution of 1:500
 |
| | |
