| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, WB, IHC, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | Q9Y5T5 |
| Gene ID | |
| Calculated MW | 112 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Specifically deubiquitinates 'Lys-120' of histone H2A(H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3(H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B-and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. |
| Immunogen | ubiquitin specific peptidase 16 |
| Synonyms | Deubiquitinating enzyme 16, Ubiquitin thiolesterase 16, UBP M, USP16 |
| Recommended dilution | WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:20-1:200 |
Immunohistochemistry of paraffin-embedded human kidney using FNab09312(USP16 antibody) at dilution of 1:100
 |
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09312(USP16 antibody) at dilution of 1:1000
 |
| | |
