| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, WB, IHC |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | Q969M7 |
| Gene ID | |
| Calculated MW | 23 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. |
| Immunogen | ubiquitin-conjugating enzyme E2F(putative) |
| Synonyms | NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme 2, NEDD8 protein ligase UBE2F, UBE2F |
| Recommended dilution | WB: 1:500-1:5000; IHC: 1:20-1:200 |
Immunohistochemistry of paraffin-embedded human skin using FNab09173(UBE2F antibody) at dilution of 1:100
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Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09173(UBE2F antibody) at dilution of 1:500
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