| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, WB, IHC, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P14373 |
| Gene ID | |
| Calculated MW | 71 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. |
| Immunogen | tripartite motif-containing 27 |
| Synonyms | Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP |
| Recommended dilution | WB: 1:500-1:2000; IP: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200 |
Immunohistochemistry of paraffin-embedded human lung cancer using FNab08977(TRIM27 antibody) at dilution of 1:100
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Jurkat cells were subjected to SDS PAGE followed by western blot with FNab08977(TRIM27 antibody) at dilution of 1:1000
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