| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse |
| Tested Application | ELISA, WB, IHC |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P40937 |
| Gene ID | |
| Calculated MW | 38 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Background | This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants. |
| Immunogen | replication factor C (activator 1) 5, 36.5kDa |
| Recommended dilution | WB: 1:500 - 1:2000; IHC: 1:50 - 1:200 |
Immunohistochemistry of paraffin-embedded human lung cancer using FNab07251(RFC5 antibody) at dilution of 1:100
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HeLa cells were subjected to SDS PAGE followed by western blot with FNab07251(RFC5 antibody) at dilution of 1:1000
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