anti- USP13 antibody

Спецификация

Clonality	monoclonal
Host	Mouse
Specificity	Human
Tested Application	ELISA, WB, IHC, IF
Delivery Time	2 to 4 working days
Clone ID	9E6
Isotype	IgG1
Form	liquid
Purification	Protein A+G purification
Purity	≥95% as determined by SDS-PAGE
Uniprot ID	Q92995
Gene ID
Calculated MW	97 kDa
Ссылка на страницу на сайте производителя	ссылка
Инструкция	PDF
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background	Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation(ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation(ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
Immunogen	ubiquitin specific peptidase 13(isopeptidase T-3)
Synonyms	Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13
Recommended dilution	WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab09309(USP13 Antibody) at dilution of 1:200
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09309(USP13 Antibody) at dilution of 1:1000

Информация для заказа

Область использования: Моноклональные антитела	Производство:	Fine Biotech
	Метод:	Антитела
	Объем:	100µg
	Кат. номер:	FNab09309
Цена (с НДС 20%):	по запросу	В корзину
Наименование: anti- USP13 antibody. Примечание: