| Clonality | monoclonal |
| Host | Mouse |
| Specificity | Human |
| Tested Application | ELISA, IF, IHC, WB |
| Delivery Time | 2 to 4 working days |
| Clone ID | 7E3 |
| Isotype | IgG1 |
| Form | liquid |
| Purification | Protein A+G purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | O00762 |
| Gene ID | |
| Calculated MW | 20 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome(APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. |
| Immunogen | ubiquitin-conjugating enzyme E2C |
| Synonyms | DJ447F3.2, UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C |
| Recommended dilution | WB: 1:500-1:5000; IHC: 1:20-1:200；IF: 1:10-1:100 |
Immunohistochemistry of paraffin-embedded human colon cancer slide using FNab09166(UBE2C Antibody) at dilution of 1:50
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HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09166(UBE2C Antibody) at dilution of 1:1000
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