anti- MSH2 antibodyСпецификация| Clonality | polyclonal | | Host | Rabbit | | Specificity | Human, Mouse, Rat | | Tested Application | ELISA, WB, IHC, IF, FC, IP | | Delivery Time | 2 to 4 working days | | Isotype | IgG | | Form | liquid | | Purification | Immunogen affinity purified | | Purity | ≥95% as determined by SDS-PAGE | | Uniprot ID | P43246 | | Gene ID | | | Calculated MW | 105 kDa | | Ссылка на страницу на сайте производителя | ссылка | | Инструкция | PDF | | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) | | Background | Component of the post-replicative DNA mismatch repair system(MMR). Forms two different heterodimers: MutS alpha(MSH2-MSH6 heterodimer) and MutS beta(MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. | | Immunogen | mutS homolog 2, colon cancer, nonpolyposis type 1(E. coli) | | Synonyms | COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2 | | Recommended dilution | WB: 1:1000-1:4000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100 | Immunohistochemistry of paraffin-embedded human colon cancer using FNab05371(MSH2 antibody) at dilution of 1:100
 | HeLa cells were subjected to SDS PAGE followed by western blot with FNab05371(MSH2 antibody) at dilution of 1:1000
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Информация для заказа| Область использования: | Производство: | Fine Biotech | | Метод: | Антитела | | Объем: | 100µg | | Кат. номер: | FNab05371 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: anti- MSH2 antibody.
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