| Clonality | monoclonal |
| Host | Mouse |
| Specificity | Human, Mouse |
| Tested Application | ELISA, IHC, WB |
| Delivery Time | 2 to 4 working days |
| Clone ID | 5H4 |
| Isotype | IgG1 |
| Form | liquid |
| Purification | Protein A+G purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Calculated MW | 60KD |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification. |
| Immunogen | v-akt murine thymoma viral oncogene homolog 1 |
| Synonyms | AKT, AKT1, AKT1-phospho-S473, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha |
| Recommended dilution |
WB: 1:1000-1:10000; IHC: 1:100-1:400 |
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06402(AKT1-phospho-S473 Antibody) at dilution of 1:200
 |
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab06402(AKT1-phospho-S473 Antibody) at dilution of 1:2000
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