anti- HIF1a antibodyСпецификация| Clonality | polyclonal | | Host | Rabbit | | Specificity | Human | | Tested Application | ELISA, IHC, IF, FC, WB | | Delivery Time | 2 to 4 working days | | Isotype | IgG | | Form | liquid | | Purification | Immunogen affinity purified | | Purity | ≥95% as determined by SDS-PAGE | | Uniprot ID | Q16665 | | Gene ID | | | Calculated MW | 120 kDa | | Ссылка на страницу на сайте производителя | ссылка | | Инструкция | PDF | | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) | | Background | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. | | Immunogen | hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor) | | Synonyms | BHLHE78, MOP1, PASD8 | | Recommended dilution | WB: 1:200-1:1000; IHC: 1:50-1:200; IF: 1:10-1:100 | Immunohistochemistry of paraffin-embedded human thyroid cancer using FNab03859(HIF1A antibody) at dilution of 1:100
 | mouse small intestine tissue were subjected to SDS PAGE followed by western blot with FNab03859(HIF1a Antibody) at dilution of 1:300
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Информация для заказа| Область использования: | Производство: | Fine Biotech | | Метод: | Антитела | | Объем: | 100µg | | Кат. номер: | FNab03859 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: anti- HIF1a antibody.
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