anti- p65/RELA antibodyСпецификация| Highlight | Pubmed/Publication | | Clonality | polyclonal | | Host | Rabbit | | Specificity | Human, Mouse, Rat, Pig | | Tested Application | ELISA, IHC, IF, IP, WB, FC | | Delivery Time | 2 to 4 working days | | Isotype | IgG | | Form | liquid | | Purification | Immunogen affinity purified | | Purity | ≥95% as determined by SDS-PAGE | | Uniprot ID | Q04206 | | Ссылка на страницу на сайте производителя | ссылка | | Инструкция | PDF | | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) | | Background | Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA. | | Immunogen | v-rel reticuloendotheliosis viral oncogene homolog A(avian) | | Synonyms | NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65 | | Recommended dilution | WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:10-1:100 | Immunohistochemistry of paraffin-embedded human liver cancer using FNab06089(p65 antibody) at dilution of 1:50
 | HeLa cells were subjected to SDS PAGE followed by western blot with FNab06089(p65 antibody) at dilution of 1:1000
 | | | |
Информация для заказа| Область использования: | Производство: | Fine Biotech | | Метод: | Антитела | | Объем: | 100µg | | Кат. номер: | FNab06089 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: anti- p65/RELA antibody.
Примечание: |
|
