| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse |
| Tested Application | ELISA, WB, IHC, IF, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P39748 |
| Gene ID | |
| Calculated MW | 42 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Background | The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. |
| Immunogen | flap structure-specific endonuclease 1 |
| Synonyms | RAD2 |
| Recommended dilution | WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:10 - 1:100; IP : 1:50 - 1:200 |
Immunohistochemistry of paraffin-embedded human lung cancer using FNab03075( FEN1 Antibody) at dilution of 1:100
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NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab03075(FEN1 antibody) at dilution of 1:1000
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