| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse |
| Tested Application | ELISA, WB, IHC, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P30305 |
| Gene ID | |
| Calculated MW | 60-70 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B). |
| Immunogen | cell division cycle 25 homolog B(S. pombe) |
| Synonyms | CDC25B, CDC25HU2, M phase inducer phosphatase 2 |
| Recommended dilution | WB: 1:200-1:1000; IP: 1:200-1:1000; IHC: 1:20-1:200 |
Immunohistochemistry of paraffin-embedded human lymphoma using FNab01522(CDC25B antibody) at dilution of 1:100
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Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01522(CDC25B antibody) at dilution of 1:300
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