| Clonality | monoclonal |
| Host | Mouse |
| Specificity | Human |
| Tested Application | ELISA, WB, IHC |
| Delivery Time | 2 to 4 working days |
| Clone ID | 2E4 |
| Isotype | IgG1 |
| Form | liquid |
| Purification | Protein A+G purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P07766 |
| Calculated MW | 23 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Background | CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule. |
| Immunogen | CD3e molecule, epsilon (CD3-TCR complex) |
| Synonyms | CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE |
| Recommended dilution | WB: 1:2000 - 1:20000; IHC: 1:200 - 1:400 |
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using FNab01459( CD3E Antibody) at dilution of 1:200
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Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01459( CD3E Antibody) at dilution of 1:10000
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