anti- beta actin antibodyСпецификация| Highlight | Pubmed/Publication | | Clonality | polyclonal | | Host | Rabbit | | Specificity | Human, Mouse, Rat | | Tested Application | ELISA, IF, WB, IHC | | Delivery Time | 2 to 4 working days | | Isotype | IgG | | Form | liquid | | Purification | Immunogen affinity purified | | Purity | ≥95% as determined by SDS-PAGE | | Uniprot ID | P60709 | | Calculated MW | 42 kDa | | Ссылка на страницу на сайте производителя | ссылка | | Инструкция | PDF | | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) | | Background | Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. | | Immunogen | actin, beta | | Synonyms | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin | | Recommended dilution | WB: 1:1000-1:10000; IHC: 1:20-1:500; IF: 1:10-1:100 | Immunohistochemistry of paraffin-embedded human heart using FNab00869(ACTB antibody) at dilution of 1:100
 | C6 cells were subjected to SDS PAGE followed by western blot with FNab00869(beta actin Antibody) at dilution of 1:2000
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Информация для заказа| Область использования: | Производство: | Fine Biotech | | Метод: | Антитела | | Объем: | 100µg | | Кат. номер: | FNab00869 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: anti- beta actin antibody.
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