| Uniprot No. |
Q86VB7 |
| Image |
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Immunofluorescence staining of Hela cells with CSB-PA801238LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
|
| Protocols |
ELISA Protocol
Immunofluorescence (IF) Protocol |
| Immunogen |
Recombinant Human Scavenger receptor cysteine-rich type 1 protein M130 protein (801-940AA) |
| Raised in |
Rabbit |
| Species Reactivity |
Human |
| Tested Applications |
ELISA, IF; Recommended dilution: IF:1:50-1:200 |
| Relevance |
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. |
| Form |
Liquid |
| Conjugate |
Non-conjugated |
| Storage Buffer |
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method |
>95%, Protein G purified |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Alias |
Scavenger receptor cysteine-rich type 1 protein M130 (Hemoglobin scavenger receptor) (CD antigen CD163) [Cleaved into: Soluble CD163 (sCD163)], CD163, M130 |
| Immunogen Species |
Homo sapiens (Human) |
| Research Area |
Immunology |
| Target Names |
CD163 |
| Storage |
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
