| Объем | 50 мкл |
| Синонимы | Thiamin pyrophosphokinase 1 (hTPK1) (EC 2.7.6.2) (Placental protein 20) (PP20) (Thiamine pyrophosphokinase 1), TPK1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H3S4 |
| Иммуноген | Recombinant Human Thiamin pyrophosphokinase 1 protein (1-243AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Thiamin pyrophosphokinase 1 |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: TPK1 antibody at 0.78µg/ml
Lane 1: Mouse liver tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28, 14 kDa
Observed band size: 28 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA024103ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA024103ESR2HU at dilution of 1:100
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Immunofluorescent analysis of PC-3 cells using CSB-PA024103ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating TPK1 in Mouse kidney tissue
Lane 1: Rabbit control IgG instead of CSB-PA024103ESR2HU in Mouse kidney tissue.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA024103ESR2HU (8µg) + Mouse kidney tissue (500µg)
Lane 3: Mouse kidney tissue (10µg)
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Наименование: TPK1 Antibody.