| Объем | 50 мкл |
| Синонимы | THO complex subunit 1 (Tho1) (Nuclear matrix protein p84) (p84N5) (hTREX84), THOC1, HPR1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q96FV9 |
| Иммуноген | Recombinant Human THO complex subunit 1 protein (428-657AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, ChIP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Tags & Cell Markers |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: THOC1 antibody at 3µg/ml
Lane 1: Jurkats whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: A549 whole cell lysate
Lane 5: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 76, 44 kDa
Observed band size: 76 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA853419ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA853419ESR1HU at dilution of 1:100
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Chromatin Immunoprecipitation 293T (1.6*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-THOC1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP853419HU) against the RHOX5 promoter.
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Наименование: THOC1 Antibody.