RELA (Ab-311) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q04206 | | Иммуноген | Synthesized non-phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 311 (F-K-S(p)-I-M). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:3000 | | Примечание | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.<br/><br/>Baeuerle, P.A. and Henkel, T. (1994) Annu. Rev. Immunol. 12, 141-179. <br/>Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. <br/>Haskill, S. et al. (1991) Cell 65, 1281-1289. <br/>Thompson, J.E. et al. (1995) Cell 80, 573-582.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Cardiovascular,Cell biology,Metabolism,Microbiology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from NIH-3T3, using NF-kappaB p65 (Ab-311) antibody.
 | Western blot analysis of extracts from cos-7 cells (Lane 2), using NF-?B p65 (Ab-311) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: RELA (Ab-311) Antibody.