Raf1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | c-RAF, RAF proto-oncogene serine/threonine-protein kinase, | | Клональность | Polyclonal Antibody | | Организм | Rat | | uniprot | P11345 | | Иммуноген | Peptide sequence around aa. 641-645(T-S-P-R-L ) derived from Rat Raf-1. | | Источник | Rabbit | | Видовая специфичность | Rat | | Применение | ELISA, WB,WB:1:500-1:1000 | | Примечание | A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to <br/><br/>Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. <br/>Chong, H. et al. (2001) EMBO J. 20, 3716-3727. <br/>King, A.J. et al. (1998) Nature 396, 180-183. <br/>Fabian, J.R. et al. (1993) Mol. Cell Biol. 13, 7170-7179. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide | | Области исследований | Cancer,Cell biology,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody
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Наименование: Raf1 Antibody.