| Объем | 50 мкг |
| Синонимы | DNA repair protein RAD51 homolog 1 (HsRAD51) (hRAD51) (RAD51 homolog A), RAD51, RAD51A RECA |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q06609 |
| Иммуноген | Recombinant Human DNA repair protein RAD51 homolog 1 protein (1-228AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | DNA repair protein RAD51 homolog 1 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate
All lanes: RAD51 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 27, 32 kDa
Observed band size: 37 kDa
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA019268LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA019268LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating RAD51 in K562 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA019268LA01HU in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA019268LA01HU (4µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)
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Наименование: RAD51 Antibody.