PTEN (Ab-370) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | MMAC1, Mutated in multiple advanced cancers 1, Protein-tyrosine phosphatase PTEN, TEP1, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P60484 | | Иммуноген | Peptide sequence around aa.368~372 (D-V-S-D-N) derived from Human PTEN. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.<br/><br/>Al-Khouri AM, et al. (2005). J Biol Chem. 280 (42):35195-35202.<br/>Miller SJ, et al. (2002). FEBS Lett. 528 (1-3): 145-153.<br/>Torres J, et al. (2001). J Biol Chem.276 (2): 993-998.<br/>Vazquez F, et al. (2000). Mol Cell Biol.20 (14): 5010-5018.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from C2C12 cells using PTEN(Ab-370) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTEN(Ab-370) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: PTEN (Ab-370) Antibody.