PRKCQ (Ab-676) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | nPKC-theta | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q04759 | | Иммуноген | Peptide sequence around aa. 674~678 (R-L-S-F-A) derived from Human PKCth. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.<br/><br/>Kristof Van Kolen, et al. (2006) FEBS J , 273: 1843 - 1854.<br/>Martin Villalba, et al. (2002) J. Cell Biol , 157: 253. <br/>Jie Zhang, et al. (2004) J. Biol. Chem , 279: 22118 - 22123. <br/>Castro AF, et al. (1998) Am J Physiol Cell Physiol, 275: C113 - C119.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from PC12 cells using PKCth(Ab-676) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Ab-676) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Ab-676) Antibody.
 | | | |
Информация для заказа |
Наименование: PRKCQ (Ab-676) Antibody.