| Объем | 100 мкл |
| Синонимы | SYK, KSYK, FLJ25043, FLJ37489, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P43405 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 352(S-P-Y(p)-A-D) derived from Human SYK. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion <br/><br/>Yagi S., Biochem. Biophys. Res. Commun. 200:28-34(1994).<br />Law C.-L., J. Biol. Chem. 269:12310-12319(1994).<br /><br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr352 |
| Области исследований | Cardiovascular,Immunology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using SYK (Phospho-Tyr352) Antibody. The lane on the left is treated with antigen-specific peptide.
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Наименование: Phospho-SYK (Tyr352) Antibody.