Phospho-SMAD2 (S255) Antibody

Спецификация

Объем	100 мкл
Синонимы	Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2
Тип антител	Recombinant Antibody
Species	Human
UniProt ID	Q15796
Иммуноген	A synthesized peptide derived from human Phospho-SMAD2 (S255)
Видовая специфичность	Human
Применение	ELISA, IF, Recommended dilution: IF:1:20-1:200
Клональность	Monoclonal
Изотип	Rabbit IgG
Коньюгат	Non-conjugated
Буффер	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Форма	Liquid
Хранение	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки	Affinity-chromatography
Области исследований	Signal Transduction
Аббревиатура	Mothers against decapentaplegic homolog 2
Примечание	Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Ссылка на страницу на сайте производителя	ссылка
Immunofluorescence staining of A549 cells with CSB-RA618017A255phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Информация для заказа

Область использования: Рекомбинантные антитела Cusabio	Производство:	Cusabio
	Метод:	Антитела
	Объем:	100 мкл
	Кат. номер:	CSB-RA618017A255phHU
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Phospho-SMAD2 (S255) Antibody. Примечание: дополнительная информация (на английском языке).