| Объем | 100 мкл |
| Синонимы | KS6B1, P70-S6K, RPS6KB1, S6K, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P23443 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 424 (P-V-S(p)-P-V) derived from Human p70 S6 Kinase. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.<br/><br/>Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851<br/>Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.<br/>Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c<br/>Le, X.F, et al. (2003) Oncogene 22: 484 |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser424 |
| Области исследований | Epigenetics and Nuclear Signaling,Cell biology,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase(Phospho-Ser424) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Ser424) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, centrosomal and nuclear staining using p70 S6 Kinase(Phospho-Ser424) Antibody.
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Наименование: Phospho-RPS6KB1 (Ser424) Antibody.