| Объем | 100 мкл |
| Синонимы | IRF3, Interferon regulatory factor 3, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14653 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 386(A-S-S(p)-L-E) derived from Human IRF-3. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.<br/><br/>Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995).<br />The MGC Project Team, Genome Res. 14:2121-2127(2004).<br />Bellingham J., Ann. Hum. Genet. 62:231-234(1998).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser386 |
| Области исследований | Epigenetics and Nuclear Signaling,Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells (Lane 2) and colo cells (Lane 3), using IRF-3 (Phospho-Ser386) Antibody. The lane on the left is treated with antigen-specific peptide.
 |
| | |
Наименование: Phospho-IRF3 (Ser386) Antibody.