MDM2 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | HDMX, hdm2, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q00987 | | Иммуноген | Peptide sequence around aa.18~22(Q-I-P-A-S) derived from Human MDM2. | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB,WB:1:500-1:1000 | | Примечание | MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .<br/><br/>Haupt, Y. et al. (1997) Nature 387, 296-299. <br/>Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. <br/>Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. <br/>Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415. <br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 and Hela cells using MDM2 Antibody.
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Наименование: MDM2 Antibody.