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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

MAPK1/MAPK3 (Ab-205/222) Antibody

Спецификация

Объем100 мкл
СинонимыERK, ERK-2, ERK2, ERT1, Extracellular signal-regulated kinase 2
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotP28482/P27361
ИммуногенSynthesized peptide derived from Internal of human MAPK1.
ИсточникRabbit
Видовая специфичностьHuman, Mouse, Rat
ПрименениеELISA, WB,WB:1:500-1:3000
Примечание<p>Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.<br /><br />Owaki H., Biochem. Biophys. Res. Commun. 182:1416-1422(1992).<br />Gonzalez F.A., FEBS Lett. 304:170-178(1992).<br />Dunham I., Nature 402:489-495(1999).</p>
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Формаliquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Области исследованийNeuroscience,Signal transduction,Stem cells
Ссылка на страницу на сайте производителяссылка
Western blot analysis of extracts from NIH-3T3 cells, using MAPK1/3 (Ab-205/222) antibody.
Western blot analysis of extracts from K562 cells (Lane 2), using MAPK1/3 (Ab-205/222) antiobdy. The lane on the left is treated with synthesized peptide.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкл 
Кат. номер:CSB-PA181879
Цена (с НДС 20%):по запросуВ корзину
MAPK1/MAPK3 (Ab-205/222) AntibodyНаименование: MAPK1/MAPK3 (Ab-205/222) Antibody.
Примечание: дополнительная информация (на английском языке).
   
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