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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

WWOX Antibody

Спецификация

Объем50 мкг
СинонимыWW domain-containing oxidoreductase (EC 1.1.1.-) (Fragile site FRA16D oxidoreductase) (Short chain dehydrogenase/reductase family 41C member 1), WWOX, FOR SDR41C1 WOX1
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotQ9NZC7
ИммуногенRecombinant Human WW domain-containing oxidoreductase protein (1-180AA)
ИсточникRabbit
Видовая специфичностьHuman, Rat
ПрименениеELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000
ПримечаниеPutative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
АбревеатураWW domain-containing oxidoreductase
Области исследованийEpigenetics and Nuclear Signaling, Neuroscience, Cancer, Cell biology
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, HEK293 whole cell lysate, Rat kidney tissue
All lanes: WWOX antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 42, 22, 5, 27, 36, 24 kDa
Observed band size: 47 kDa

IHC image of CSB-PA873704LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA873704LA01HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CSB-PA873704LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating WWOX in Rat kidney tissue
Lane 1: Rabbit control IgG instead of CSB-PA873704LA01HU in Rat kidney tissue. For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA873704LA01HU (8µg) + Rat kidney tissue (500µg)
Lane 3: Rat kidney tissue (10µg)

Immunofluorescence staining of MCF-7 cells with CSB-PA873704LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкг 
Кат. номер:CSB-PA873704LA01HU
Цена (с НДС 20%):по запросуВ корзину
WWOX AntibodyНаименование: WWOX Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43