Histone H3 (Ab-128) AntibodyСпецификация| Объем | 50 мкл | | Синонимы | Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P68431 | | Иммуноген | Peptide sequence around site of Mono-methyl-Arg (128) derived from Human Histone H3.1 | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:200-1:2000, IHC:1:10-1:100, IP:1:200-1:2000 | | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Абревеатура | Histone H3.1 | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | IHC image of CSB-PA010418OA128nme1HU diluted at 1:20 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
 | IHC image of CSB-PA010418OA128nme1HU diluted at 1:20 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse brain tissue, Mouse kidney tissue
All lanes: HIST1H3A antibody at 0.53µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
 | Immunoprecipitating HIST1H3A in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010418OA128nme1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010418OA128nme1HU (5µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 50 мкл | | Кат. номер: | CSB-PA010418OA128nme1HU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Histone H3 (Ab-128) Antibody.
Примечание: дополнительная информация (на английском языке). |
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