| Объем | 100 мкг |
| Синонимы | Alanine aminotransferase 1 (ALT1) (EC 2.6.1.2) (Glutamate pyruvate transaminase 1) (GPT 1) (Glutamic--alanine transaminase 1) (Glutamic--pyruvic transaminase 1), GPT, AAT1 GPT1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P24298 |
| Иммуноген | Recombinant Human Alanine aminotransferase 1 protein (2-496AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Alanine aminotransferase 1 antibody at 20µg/ml
Lane 1: Rat heart tissue
Lane 2: Rat liver tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA009864LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA009864LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue
All lanes: GPT antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
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IHC image of CSB-PA009864LA01HU diluted at 1:350 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating GPT in Mouse liver tissue
Lane 1: Rabbit control IgG instead of CSB-PA009864LA01HU in Mouse liver tissue.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA009864LA01HU (8µg) + Mouse liver tissue (500µg)
Lane 3: Mouse liver tissue (10µg)
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Наименование: GPT Antibody.